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Increasing serum concentrations decrease cell death and allow growth of hybridoma cells at higher agitation rates

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Summary

Hybridoma cells CRL-8018 were grown in a well controlled 1.2 l bioreactor at high agitation rates (220 rpm) with fetal bovine serum (FBS) concentrations varying between 1% and 10%. No net growth was observed at FBS concentrations below 5%, while higher FBS concentrations allowed faster growth. The inferred death rate shows a continuous decrease with FBS concentrations. 10% FBS allows cell growth even at 280 rpm in our bioreactor. A simple model is proposed for the quantitative description of the FBS effect on cell growth and death.

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Authors and Affiliations

  1. Department of Chemical Engineering, Rice University, P. O. Box 1892, 77251-1892, Houston, TX, USA

    K. T. Kunas

  2. Department of Chemical Engineering, Northwestern University, 60208-3120, Evanston, IL, USA

    E. T. Papoutsakis

Authors
  1. K. T. Kunas
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  2. E. T. Papoutsakis
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Kunas, K.T., Papoutsakis, E.T. Increasing serum concentrations decrease cell death and allow growth of hybridoma cells at higher agitation rates. Biotechnol Lett 11, 525–530 (1989). https://doi.org/10.1007/BF01040029

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