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Development of media for growth ofDioscorea Deltoidea cells andin vitro diosgenin production: Influence of media constituents and nutrient stress

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Summary

Callus culture ofDioscorea deltoidea produced diosgenin and sterols during stationary phase. Ammonium nitrate (420 mg Nitrogen/l) as sole nitrogen source supported better growth than a combination of ammonium nitrate and potassium nitrate (totally equivalent to 840 mg Nitrogen/l). The production of diosgenin increased under low phosphate concentration (100 mg/l) whereas high phosphate concentration (240 mg/l) promoted growth. Micronutrients, when used at 1 1/2 strength, enhanced growth and diosgenin production. Depletion of nitrogen increased the diosgenin synthesis by a factor of 2. Adoption of a two stage culture method enhanced the diosgenin production in cultured cells by eight-fold.

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Authors and Affiliations

  1. Autotrophic Cell Culture Discipline, Central Food Technological Research Institute (CSIR), 570 013, Mysore, India

    G. A. Ravishankar

  2. Plant Tissue Culture Lab, Regional Research Laboratory (CSIR), 180 001, Jammu-Tawi, India

    S. Grewal

Authors
  1. G. A. Ravishankar
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  2. S. Grewal
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Ravishankar, G.A., Grewal, S. Development of media for growth ofDioscorea Deltoidea cells andin vitro diosgenin production: Influence of media constituents and nutrient stress. Biotechnol Lett 13, 125–130 (1991). https://doi.org/10.1007/BF01030463

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  • DOI: https://doi.org/10.1007/BF01030463

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