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Separation of trypsin from trypsin α-chymotrypsin mixture by affinity-ultrafiltration

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Summary

Water-sobuble trypsin specific macroligands were prepared to separate the trypsin α-chymotrypsin mixture with affinity-ultrafiltration technique. Soya bean trypsin inhibitor (STI) attached to cyanogen bromideactivated Dextran showed a good selectivity and low non-specific adsorption properties. The experiments performed with STI-Dextran polymer gave a 81% purified trypsin from 50%-50% mixture.

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Author notes

  1. T. B. Choe

    Present address: Genetic Engineering Center, Korea Advanced Institute of Science, Chungyangni, Po Box 150, SEOUL, Korea

Authors and Affiliations

  1. Laboratoire de Chimie Organique Appliquée, Institut National des Sciences Appliquées, Av. de rangueil, 31077, Toulouse cedex, FRANCE

    T. B. Choe, P. Massé & A. Verdier

Authors
  1. T. B. Choe
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  2. P. Massé
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  3. A. Verdier
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Choe, T.B., Massé, P. & Verdier, A. Separation of trypsin from trypsin α-chymotrypsin mixture by affinity-ultrafiltration. Biotechnol Lett 8, 163–168 (1986). https://doi.org/10.1007/BF01029372

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  • DOI: https://doi.org/10.1007/BF01029372

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