Skip to main content
SpringerLink
Log in

Release of β-galactosidase from E. coli by a plasmid containing a temperature sensitive lytic function

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

We report the construction of a plasmid which carries the øX174 lysis gene E downstream from the lambda pL promoter. This plasmid is capable of triggering lysis inE. coli when the growth temperature is raised from 30°C to 42°C. The kinetics of release of β-galactosidase and intracellular protein have been determined, as well as the decrease in cell viability upon induction of lysis. In addition, β-galactosidase can be released efficiently after induction by a short sonication period. Both methods allow efficient release of β-galactosidase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Bibliography

  • Auerbach, J. and M. Rosenberg. (1987). U.S. Patent # 4, 637, 980.

  • Bradford, M. (1976). Anal. Biochem. 72:248.

    Google Scholar 

  • Garret, J., J. Fusselman, L. Chiou, D. Smith-Grillo, J. Schultz, and R. Young. (1981). Mol. Gen. Gen. 182:326.

    Google Scholar 

  • Henrich, B. and R. Plapp. (1984). J. Biochem. & Biophys. Methods 10:25.

    Google Scholar 

  • Maniatis, T., E.F. Fritsch, J. Sambrook. (1982). Molecular Cloning: A Laboratory Manual. pg. 68. Cold Spring Harbor Laboratory, New York.

    Google Scholar 

  • Markert, A., W. Zillig. (1965). Vir. 25:88–97.

    Google Scholar 

  • Miller, J.H. (1972). Experiments in Molecular Genetics., 3rd Ed., pp. 352–355, Cold Spring Harbor Laboratory, New York.

    Google Scholar 

  • Rosenberg, M., Y. Ho, A. Shatzman. (1983). Methods in Enzymology 101. pg. 123. Academic Press, Orlando.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Applied Biological Sciences, Massachusetts Institute of Technology, 02139, Cambridge, MA

    R. L. Dabora & C. L. Cooney

  2. Department of Biological Sciences, Univ. of Lowell, 01854, Lowell, MA

    D. T. Eberiel

Authors
  1. R. L. Dabora
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. T. Eberiel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. L. Cooney
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dabora, R.L., Eberiel, D.T. & Cooney, C.L. Release of β-galactosidase from E. coli by a plasmid containing a temperature sensitive lytic function. Biotechnol Lett 11, 845–850 (1989). https://doi.org/10.1007/BF01026838

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01026838

Keywords

Search

Navigation