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High cell density culture of E. coli in a fed-batch system with dissolved oxygen as substrate feed indicator

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Summary

A fed batch system using the dissolved oxygen concentration as substrate feed indicator has been developed to perform high cell density E. coli culture. A cell density of about 110 g/l (dw) has been obtained within 12 hours with an overall growth yield of about 0.68 gX/gGlucose.

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Authors and Affiliations

  1. L'Air Liquide-Centre de recherche Claude Delorme, BP 126, F 78350, Les Loges En Josas, France

    J M Cutayar & D Poillon

Authors
  1. J M Cutayar
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  2. D Poillon
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Cutayar, J.M., Poillon, D. High cell density culture of E. coli in a fed-batch system with dissolved oxygen as substrate feed indicator. Biotechnol Lett 11, 155–160 (1989). https://doi.org/10.1007/BF01026048

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  • DOI: https://doi.org/10.1007/BF01026048

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