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The continuous hydrolysis of geniposide to genipin using immobilized β-glucosidase on calcium alginate gel

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Summary

The reaction velocity of immobilized β-glucosidase was approximated by the first-order reaction kinetics. A plug flow reactor was used for continuous hydrolysis of geniposide with this immobilized enzyme. The activity of this immobilized enzyme was retained 100% for 600 h. The amount of genipin formed by using the immobilized enzyme was 17 fold that formed using the native enzyme without reuse. Using immobilized enzyme, purity and yield of genipin, which is a hydrolyzate of geniposide, was improved comparing with the native enzyme.

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Authors and Affiliations

  1. Research Lab. of Alcoholic Beverages and Fermentation Technology, Suntory Ltd., Shimamoto-cho, Mishima-gun, 618, Osaka, Japan

    Shigeaki Fujikawa, Tomoko Yokota, Kunimasa Koga & Jun-ichi Kumada

Authors
  1. Shigeaki Fujikawa
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  2. Tomoko Yokota
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  3. Kunimasa Koga
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  4. Jun-ichi Kumada
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Fujikawa, S., Yokota, T., Koga, K. et al. The continuous hydrolysis of geniposide to genipin using immobilized β-glucosidase on calcium alginate gel. Biotechnol Lett 9, 697–702 (1987). https://doi.org/10.1007/BF01024600

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  • DOI: https://doi.org/10.1007/BF01024600

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