Summary
ThelysC gene encoding aspartokinase of a lysine-hyperproducing mutantBrevibacterium flavum CCRC 18271 was cloned by polymerase chain reaction and its expression in CCRC 18271 was studied. Enzyme assays showed thatB. flavum cells harbouring thelysC gene exhibited 4- to 11-fold higher specific activities of aspartokinase as compared to respective hosts. Introduction of thelysC gene intoB. flavum CCRC 18271 resulted in increased lysine production up to 33% in flask fermentations.
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Lu, JH., Chen, JL. & Liao, CC. Molecular breeding of aBrevibacterium flavum L-lysine producer using a cloned aspartokinase gene. Biotechnol Lett 16, 449–454 (1994). https://doi.org/10.1007/BF01023324
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DOI: https://doi.org/10.1007/BF01023324