Abstract
Electroantennograms (EAGs) of unmated laboratory-reared male and femaleCeratitis capitata (Wiedemann) were recorded in response to the attractant trimedlure [tert-butyl 4(and 5)-chloro-trans-2-methylcyclohexane-1-carboxylate] and its fourtrans isomers. For both sexes, the magnitude of the EAG response was relatively low as compared to other previously tested compounds (i.e., plant volatiles). Dosage-response curves generated for all TML isomers revealed that flies responded to increasing dosages over a relatively narrow range (two to three log steps). Responses for both sexes peaked at ca. 10 μg dose for all isomers. Antennal response in males was greatest to the C isomer followed by the B1, A, and B2 isomers, while responses of females were greatest for the A isomer followed by B1, C, and B2. Both sexes exhibited a long recovery period for the response potential to return to baseline at doses above 1 μg for all of the isomers tested, except for B2. The low EAG sensitivity to trimedlure and the apparent EAG selectivity to the C isomer in males are discussed in relation to the known field attractancy of males to the C, A, B1, and B2 isomers.
Similar content being viewed by others
References
Beroza, M., andGreen, N. 1963. Materials tested as insect attractants. U.S. Dept. Agric. Handbook No. 239, pp. 1–134.
Beroza, M., Green, N., Gertler, S.I., Steiner, L.F., andMiyashita, D.N. 1961. New attractants for the Mediterranean fruit fly.J. Agric. Food Chem. 9:361–365.
Blaney, W.M., Schoonhoven, L.M., andSimmonds, M.S.J. 1986. Sensitivity variations in insect chemoreceptors; a review.Experientia 42:13–19.
Davis, E.E. 1984. Regulation of sensitivity in the peripheral chemoreceptor systems for host seeking behavior by a hemolymph-borne factor inAedes aegypti.J. Insect Physiol. 30:179–183.
Dickens, J.C. 1984. Olfaction in the boll weevil,Anthonomus grandis Boh. (Coleoptera: Curculionidae): Electroantennogram studies.J. Chem. Ecol. 10:1759–1785.
Dickens, J.C., Hart, W.G., Light, D.M., andJang, E.B. 1988. Tephritid olfaction: Morphology of the antennae of four tropical species of economic importance.Ann. Entomol. Soc. Am. 81:325–331.
Duncan, D.B. 1955. Multiple range and multiple F tests.Biometrics 11:1–41.
Fein, B.L., Reissig, W.H., andRoelofs, W.L. 1982. Identification of apple volatiles attractive to the apple maggot,Rhagoletis pomonella.J. Chem. Ecol. 8:1473–1487.
Gertler, S.I., Steiner, L.F., Mitchell, W.C., andBarthel, W.F. 1958. Esters of 6-methyl- 3-cyclohexene-1-carboxylic acid as attractants for the Mediterranean fruit fly.J. Agric. Food Chem. 6:592–594.
Guerin, P.M., Remund, U., Boller, E.F., Katsoyannos, B., andDelrio, G. 1983. Fruit fly electroantennogram and behavior responses to some generally occurring fruit volatiles, pp. 248–251,in R. Cavalloro (ed.). Proceedings, CEC and IOBC International Symposium on Fruit Flies of Economic ImportanceAthens, Greece. A.A. Balkema, Rotterdam.
Jang, E.B., Light, D.M., Flath, R.A., Nagata, J.T., andMon, T.R. 1989. Electroantennogram responses of the Mediterranean fruit fly,Ceratitis capitata to identified volatile constituents from calling males.Entomol. Exp. Appl. 50:7–19.
Kaissling, K.E. 1969. Kinetics of olfactory receptor potentials, pp. 52–70,in C. Pfaffmann (ed.). III International Symposium on Olfaction and Taste. Rockefeller University Press, New York.
Kaissling, K.E. 1971. Insect Olfaction, pp. 351–431,in L.M. Beidler (ed.). Handbook of Sensory Physiology, Vol. IV/I. Springer-Verlag, Berlin.
Kaissling, K.E. 1974. Sensory transduction in insect olfactory receptors, pp. 243–273,in L. Jaenicke (ed.). Biochemistry of Sensory Functions. Springer Verlag, Berlin.
Kaissling, K.E. 1986. Chemo-electrical transduction in insect olfactory receptors.Annu. Rev. Neurosci. 9:121–145.
Keiser, I., Harris, E.J., andMiyashita, D.H. 1975. Attraction of ethyl ether extracts of 232 botanicals to oriental fruit flies, melon flies, and Mediterranean fruit flies.Lloydia 31:141–152.
Leonhardt, B.A., McGovern, T.P., andPlimmer, J.R. 1982. Capillary GC analysis of trimedlure, the attractant for the “Medfly.”J. High Resol. Chromatogr. Chromatogr. Commun. 5:430–431.
Light, D.M. 1983. Sensitivity of antennae of male and femaleIps paraconfusus (Coleoptera: Scolytidae) to their natural aggregation pheromone and its enantiomeric components.J. Chem. Ecol. 9:561–583.
Light, D.M., andJang, E.B. 1987. Electroantennogram responses of the Oriental fruit fly,Dacus dorsalis, to a spectrum of alcohol and aldehyde plant volatiles.Entomol. Exp. Appl. 45:55–64.
Light, D.M., Jang, E.B., andDickens, J.C. 1988. Electroantennogram responses of the Mediterranean fruit fly,Ceratitis capitata, to a spectrum of plant volatiles.J. Chem. Ecol. 14:159–180.
Mankin, R.W., andMayer, M.S. 1983. Stimulus-response relationships of insect olfaction: Correlations among neurophysiological and behavioral measures of response.J. Theor. Biol. 100:613–630.
Mayer, M.S., Mankin, R.W., andGrant, A.J. 1987. Quantitative comparison of behavioral and neurophysiological responses of insects to odorants: Inferences about central nervous system processes.J. Chem. Ecol. 13:509–531.
McGovern, T.P., andBeroza, M. 1966. Structure of the four isomers of the insect attractant trimedlure.J. Org. Chem. 31:1472–1477.
McGovern, T.P., Beroza, M., Ohinata, K., Miyashita, D., andSteiner, L.F. 1966. Volatility and attractiveness to the Mediterranean fruit fly of trimedlure and its isomers, and a comparison of its volatility with that of seven other insect attractants.J. Econ. Entomol. 59:1450–1455.
McGovern, T.P., Cunningham, R.T., andLeonhardt, B.A. 1986. cis-Trimedlure: Attraction for the Mediterranean fruit fly (Diptera: Tephritidae) and isomeric structural assignments.J. Econ. Entomol. 79:98–102.
McGovern, T.P., Cunningham, R.T., andLeonhardt, B.A. 1987. Attractiveness oftrans-trimedlure and its four isomers in field tests with the Mediterranean fruit fly (Diptera: Tephritidae).J. Econ. Entomol. 80:617–620.
Nagai, T. 1981. Electroantennogram response gradient on the antennae of the European corn borer,Ostrinia nubildis.J. Insect Physiol. 27:889–894.
Nagai, T. 1983. On the relationship between the electroantennogram and simultaneously recorded single sensillum response of the European corn borer,Ostrinia nubildis.Arch. Insect Biochem. Physiol. 1:85–91.
Nakagawa, S., Steiner, L.F., andFarias, G.J. 1981. Response of virgin female Mediterranean fruit flies to live mature normal males, sterile males and trimedlure in plastic traps.J. Econ. Entomol. 74:566–567.
Payne, T.L. 1975. Bark beetle olfaction. III. Antennal olfactory responsiveness ofDendroctonus frontalis Zimmerman andD. brevicomis Le Conte (Coleoptera: Scolytidae) to aggregation pheromones and host tree terpene hydrocarbons.J. Chem. Ecol. 1:233–242.
Robacker, D.C., Chapa, B.E., andHart, W.G. 1986. Electroantennograms of Mexican fruit flies to chemicals produced by males.Entomol. Exp. Appl. 40:123–127.
Roelofs, W.L., Comeau, A., andSelle, R. 1969. Sex pheromone of the oriental fruit moth.Nature 224:723.
Snedecor, G.W., andCochran, W.G. 1967. Statistical Methods, 6th ed. Iowa State University Press, Ames, Iowa.
Van Der Pers, J.N.C., Haniotakis, G.E., andKing, B.M.1984. Electroantennogram responses from olfactory receptors inDacus oleae.Entomol. Hell. 2:47–53.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jang, E.B., Light, D.M., Dickens, J.C. et al. Electroantennogram responses of mediterranean fruit fly,Ceratitis capitata (Diptera: Tephritidae) to trimedlure and itstrans isomers. J Chem Ecol 15, 2219–2231 (1989). https://doi.org/10.1007/BF01014111
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01014111