Abstract
The red-necked longicorn beetle Aromia bungii (Faldermann) (Coleoptera: Cerambycidae) is an emerging invader in Japan and has become a major pest of cherry blossom trees, peaches, prunes and Japanese apricots. The male-produced aggregation-sex pheromone, recently identified as (E)-2-cis-6,7-epoxynonenal, has two enantiomers. Here, we determined which enantiomer the male produces and tested the responses of beetle antennae to the enantiomers using electroantennography. The male produced only the (6R,7S)-enantiomer, but both enantiomers elicited responses from the antennae of female beetles. Antennae of males also responded to both enantiomers. In field trials, the (6R,7S)-enantiomer and the racemic mixture were equally attractive to both sexes of the beetle, whereas the unnatural (6S,7R)-enantiomer did not attract any beetles.
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Acknowledgements
Many thanks are due to Prof. J. G. Millar of University of California, Riverside, for valuable suggestions and improving our manuscript. We thank “Kubiaka-tsuya-kamikiri busters” organized by Tokushima Prefecture to collect and count the beetles. MF and HY thank the Japan Society for the Promotion of Science (JSPS) KAKENHI (#15K07327), and HY, NF-T and TY thank JSPS KAKENHI (#17K07686) for financial support of this work. This research was also partially supported by Grants from the Project of the NARO Bio-oriented Technology Research Advancement Institution (Research program on development of innovative technology).
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Yasui, H., Fujiwara-Tsujii, N., Yasuda, T. et al. Electroantennographic responses and field attraction of an emerging invader, the red-necked longicorn beetle Aromia bungii (Coleoptera: Cerambycidae), to the chiral and racemic forms of its male-produced aggregation-sex pheromone. Appl Entomol Zool 54, 109–114 (2019). https://doi.org/10.1007/s13355-018-0600-x
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DOI: https://doi.org/10.1007/s13355-018-0600-x