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In vivo responses of macrophages and myofibroblasts in the healing following isoproterenol-induced myocardial injury in rats

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Abstract

To clarify the relation between macrophage and myofibroblast involvement in various myocardial diseases, the authors investigated the kinetics of these cells in the healing (scar tissue formation) following isoproterenol-induced myocardial injury in rats. Alphasmooth muscle actin (α-SMA) expressing myofibroblasts were seen at the border of the affected area and appeared in the greatest numbers on days 3–7 post-injection, followed by a gradual decrease by day 35. The peak on day 3 was consistent with the timing of the highest proliferative activity of myofibroblasts. The number of ED1-positive macrophages began to increase as early as day 1, reaching a peak on day 3 within the injured myocardium. The expansion of EDI-positive macrophages preceded an increased number of α-SMA-positive myofibroblasts suggesting that myofibroblast proliferation and activation may be mediated by factors released by ED1-positive mcrophages in response to myocardial injury. The number of ED2-positive tissue-fixed, resident macrophages gradually increased from day 3 post-injection, and peaked on day 14, but the number of ED2-positive macrophages was consistently fewer than that of ED1-positive macrophages during the 35 day-observation period after the injection. The labelling index of the ED2-positive cells was maximal on day 14, indicative of local proliferation of resident macrophages. In the healing process after myocardial injury, EDI-positive macrophages increase markedly in the early stages; ED2-positive macrophages appear later.

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Authors and Affiliations

  1. Laboratory of Toxicologic Pathology, Pharmaceuticals Research Center, Kanebo Limited, 1-5-90 Tomobuchi-cho, 534, Miyakojima-ku, Osaka, Japan

    Shunji Nakatsuji

  2. Department of Veterinary Pathology, College of Agriculture, University of Osaka Prefecture, Osaka, Japan

    Jyoji Yamate, Mitsuru Kuwamura, Takao Kotani & Sadasige Sakuma

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  1. Shunji Nakatsuji
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  2. Jyoji Yamate
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  3. Mitsuru Kuwamura
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  4. Takao Kotani
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  5. Sadasige Sakuma
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Nakatsuji, S., Yamate, J., Kuwamura, M. et al. In vivo responses of macrophages and myofibroblasts in the healing following isoproterenol-induced myocardial injury in rats. Virchows Archiv 430, 63–69 (1997). https://doi.org/10.1007/BF01008018

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  • DOI: https://doi.org/10.1007/BF01008018

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