Skip to main content
SpringerLink
Log in

Resistance of aldehyde blockades to periodic acid

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Synopsis

Densitometric studies on rat liver, myelin and intestinal mucin showed that, among eight groups of reagents tested, only phenylhydrazines and aniline-acetic acid reliably blockaded aldehydes in such a way as to be reasonably resistant to re-oxidation by periodic acid. It is proposed, therefore, that only these agents among those tested should be used to block pre-existing aldehydes before performing the PAS reaction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adams, C. W. M. &Bayliss, O. B. (1963). Histochemical observations on the localisation and origin of sphingoyelin, cerebroside and cholesterol in the normal and atherosclerotic human artery.J. Path. Bact. 85, 113–19.

    Google Scholar 

  • Culling, C. F. A. (1974).Handbook of Histopathological and Histochemical Techniques, 3rd Edn, p. 289 London: Butterworths.

    Google Scholar 

  • Danielli, J. F. (1949). A critical study of techniques for the cytochemical demonstration of aldehydes.Q. Jl. microsc. Sci. 90, 67–74.

    Google Scholar 

  • Peulgen, R. &Voit, K. (1924). Ueber einen Weitverbreiteten festen Aldehyd seine Entstehung auseiner Varstufe, sein mikrochemischer Nachweis und die Wege zu seiner praparativen Darstellung,Pflügers. Arch. ges. Physiol. 206, 389–410.

    Google Scholar 

  • Geyer, G. (1963). Histochemische Reduktion von aldehyden mit Natriumborohydrid.Acta histochem. 15, 1–5.

    Google Scholar 

  • Jobst, C. &Horváth, A. (1961). Contributory data on the reduction of oxo-groups in lipids.J. Histochem. Cytochem. 9, 711–12.

    Google Scholar 

  • Lillie, R. D. (1954).Histopathologic Technic and Practical Histochemistry, 2nd Edn, p. 159. New York: Blakiston.

    Google Scholar 

  • Lillie, R. D. &Glenner, G. G. (1957). Histochemical aldehyde blockade by aniline in glacial acetic acid.J. Histochem. Cytochem. 5, 167–9.

    Google Scholar 

  • Lillie, R. D. &Pizzolato, P. (1972). Histochemical use of borohydrides as aldehyde blocking reagents.Stain Technol. 47, 13–16.

    Google Scholar 

  • Oster, K. A. &Mulinos, M. G. (1944). Tissue aldehydes and their reaction with amines.J. Pharmacol. 80, 132–8.

    Google Scholar 

  • Pharse, A. G. E. (1968).Histochemistry, Theoretical and Applied, 3rd Edn, Vol. I, pp. 459, 706. London: Churchill.

    Google Scholar 

  • Staple, P. H. (1958). Effect of periodic acid on histochemical aldehyde blockades.Nature, Lond. 181, 288.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, Guy's Hospital Medical School, London Bridge, London, UK

    Olga B. Bayliss & C. W. M. Adams

Authors
  1. Olga B. Bayliss
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. W. M. Adams
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bayliss, O.B., Adams, C.W.M. Resistance of aldehyde blockades to periodic acid. Histochem J 8, 71–75 (1976). https://doi.org/10.1007/BF01004007

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01004007

Keywords

Search

Navigation