Summary
3H-tyrosine was administered to cats as a continuous i.v. infusion of 30 min duration after inhibition of DOPA-decarboxylase by N-methyl-N′(3-hydroxybenzyl) hydrazine dihydrogen phosphate (= NSD-1034). Subsequently, the content of3H-DOPA in the arterial blood, adrenal glands and adrenal venous blood was measured. For this purpose DOPA was separated from tyrosine and other radioactive tyrosine catabolites by ion exchange chromatography after adsorption onto and elution from alumina.
3H-DOPA was not only detectable in the adrenal glands and in the adrenal venous blood but also in the arterial blood. The content of DOPA in the arterial blood increased linearly during the 30 min infusion period and was 0.5 mμmole/ml at the end of the experiment. This corresponds to a DOPA content in the total blood volume of about 27.5 mμmole/kg body weight. Both adrenals synthesized 0.3 mμmole DOPA per minute and per kg body weight. The significance of the formation of a DOPA pool for the therapeutic use of DOPA decarboxylase inhibitors is discussed.
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Herrn Prof. Dr. W. Maurer danken wir für die stets großzügige Förderung der Arbeit. Fräulein Regina Kreutz, Frau Susanne Schmidt und Herrn Abdallah Ahmed El Hindy sind wir für interessierte Mitarbeit zu Dank verpflichtet. NSD-1034 wurde freundlicherweise von Smith & Nephew, Research Ltd., Harlow, England, zur Verfügung gestellt. Der Deutschen Forschungsgemeinschaft danken wir für die Gewährung von Sach- und Personalmitteln.
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Männl, H.F.K., Lange, H.W. & Hempel, K. Entstehung eines DOPA-pools bei Blockierung der DOPA-Decarboxylase. Naunyn-Schmiedebergs Arch. Pharmak. 266, 50–60 (1970). https://doi.org/10.1007/BF00997782
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DOI: https://doi.org/10.1007/BF00997782