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Histamine and histamine methyltransferase in the gastric mucosa of man, pig, dog and cow

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Summary

High histamine concentrations and histamine methyl transferase activity were demonstrated in the gastric mucosa of man, dog, pig and cow. Modified methods for the determination of histamine and histamine methyltransferase were developed. Histamine was identified by its fluorescence spectrum, by thin-layer chromatography in 8 different solvent systems and by bioassay. Histamine methyltransferase from pig antral mucosa was purified 6-fold by ultra-centrifugation and fractional precipitation by ammonium sulfate. ItsK m for histamine as substrate was 2.3×10−5 M, for S-adenosylmethionine 4.3×10−5 M, the pH-optimum was found to be pH 7.4. Nicotinamide in concentrations up to 1×10−2 M had no effect on the activity of the enzyme.

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Authors and Affiliations

  1. Institut für Klinische Chemie, Klinische Biochemie der UniversitÄt München, Deutschland

    W. Lorenz, H. Barth & E. Werle

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  1. W. Lorenz
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  2. H. Barth
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  3. E. Werle
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We thank the Deutsche Forschungsgemeinschaft for a grant (Sonderforschungs-bereich 37).

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Lorenz, W., Barth, H. & Werle, E. Histamine and histamine methyltransferase in the gastric mucosa of man, pig, dog and cow. Naunyn-Schmiedebergs Arch. Pharmak. 267, 421–432 (1970). https://doi.org/10.1007/BF00997278

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  • DOI: https://doi.org/10.1007/BF00997278

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