Skip to main content
SpringerLink
Log in

Biochemical screening test with14C-glucose and32P-phosphate for the evaluation of embryotoxic effects in vivo

  • Published:
Naunyn-Schmiedebergs Archiv für Pharmakologie Aims and scope Submit manuscript

Summary

A biochemical test system is described, which allows an evaluation of the effect of embryotoxic substances on some main metabolic pathways in vivo.

After pretreatment with the drug to be tested experimental animals—rats, mice, or other suitable species—receive an intravenous injection of both14C-glucose and32P-ortho-phosphate. These “unspecific precursors” are incorporated into a variety of acid-insoluble cell components by various metabolic pathways. Physiological concentrations of glucose or inorganic phosphate are changed to a negligible degree by the injection. The isotopes injected, therefore, do not cause by themselves an alteration in the concentration of any of the intermediary products of cell metabolism. The radioactivity incorporated into lipids, RNA, glycosaminoglycans, DNA, and proteins is measured, using a simple fractionation procedure and a double labelling technique. The applicability as well as the limitations of the method are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beck, F., Lloyd, J.B.: Methods for the study of embryotrophic nutrition. Lab. Anim.2, 157–170 (1968).

    Google Scholar 

  • Bochert, G., Mewes, P., Krowke, R., Engels, K.: Incorporation of14C-Glucose and32P-Phosphate into bases and nucleotides of nucleic acids in 11–13-day-old rat embryos. (To be published in this journal 1972).

  • Burton, K.: A study of the conditions and mechanisms of the reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J.62, 315–323 (1956).

    Google Scholar 

  • Dunn, A., Chenoweth, M., Schaeffer, L. D.: Estimation of Glucose turnover and the Cory-cycle using glucose-6-T-14C. Biochemistry6, 6–11 (1967).

    Google Scholar 

  • Hayashi, T. T., Kazmierowski, D.: Placental and fetal RNA-Metabolism. I. In-vivo labelling of the fetal and placental RNA in the 14-day pregnant rat. Gynec. Invest.1, 31–45 (1970).

    Google Scholar 

  • Katz, J., Dunn, A.: Glicose-2-T as a tracer for glucose metabolism. Biochemistry6, 1–5 (1967).

    Google Scholar 

  • Köhler, E., Merker, H. J., Ehmke, W., Wojnorowicz, F.: Growth Kinetics of mammalian embryos during the stage of differentiation. Naunyn-Schmiedebergs Arch. Pharmak. (in press) (1971).

  • Krowke, R., Neubert, D.:14C-Glucose as a precursor of nucleic acids for studying embryotoxic effects in-vivo. In: Abstracts of the 4th International Congress on Pharmacology, p. 469, Basel 1969.

  • - Pielsticker, K., Siebert, G.: A simple fractionation procedure for studying the incorporation of radioactively labelled precursors into mammalian main cell components. Naunyn-Schmiedebergs Arch. Pharmak. (in press) (1971a).

  • Krowke, R., Bochert, G., Mewes, P.: Effects of some metabolic inhibitors on nucleic acid synthesis in early stages of mammalian embryonic development. Acta pharmacol. (Kbh.)29, Suppl. 4, 27 (1971b).

    Google Scholar 

  • —, Siebert, G., Neubert, D.: Effect of 5-Fluorodeoxycytidin, 5-Fluorouracil and Cycloheximide on the incorporation of14C-glucose and32P into different cell components of rat embryos in vivo. Naunyn-Schmiedebergs Arch. Pharmak.268, 229–234 (1971c).

    Google Scholar 

  • Lloyd, J. B., Beck, F.: Evidence for a mechanism of teratogenesis involving inhibition of embryotrophic nutrition. Lab. Anim.2, 171–180 (1968).

    Google Scholar 

  • Neubert, D., Merker, H. J., Köhler, E., Krowke, R., Barrach, H.-J.: Biochemical aspects of teratology. Symposium on: Intrinsic and extrinsic factors in early mammalian development, Venice 1970. Advanc. Biosciences, Vol. VI, 575–621. Vieweg-Pergamon Press 1971.

  • Ullberg, S., Koransky, W.: Autoradiographische Untersuchungen an Schnitten durch ganze Tiere über die Verteilung markierter Substanzen. Naunyn-Schmiedebergs Arch. Pharmak.246, 65–66 (1963).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abt. „Embryonal-Pharmakologie“, Pharmakologisches Institut der Freien Universität Berlin, Germany

    Ralf Krowke, Gabriele Siebert & Diether Neubert

Authors
  1. Ralf Krowke
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gabriele Siebert
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Diether Neubert
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was generously supported by grants from the Deutsche Forschungsgemeinschaft given to the Sonderforschungsbereich 29, “Embryonale Entwicklung und Differenzierung (Embryonal-Pharmakologie)”.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Krowke, R., Siebert, G. & Neubert, D. Biochemical screening test with14C-glucose and32P-phosphate for the evaluation of embryotoxic effects in vivo. Naunyn-Schmiedebergs Arch. Pharmak. 271, 274–288 (1971). https://doi.org/10.1007/BF00997221

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00997221

Key words

Search

Navigation