Abstract
Since free sterol excretory-secretory (E-S) products are involved in pheromonal communication in adultEchinostoma revolutum (Trematoda), attempts were made to associate specific systems with the release of lipids from this organism. A micropipet design was used to isolate neutral lipids from the excretory system versus those obtained from both the alimentary and the reproductive systems. Tegumentary lipids were obtained by rubbing the surface of worms with gauze. As determined by thin-layer chromatography, the major neutral lipid obtained from all systems was free sterol. Additional minor neutral lipid fractions were obtained from the excretory, alimentary, and reproductive systems. Histochemical oil red O studies showed neutral lipids only in the excretory system. Neutral lipids released from all of the above-mentioned systems may play a role in pheromonal communication in this species.
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References
Bailey, R.S., Jr., AndFried, B. 1977. Thin layer chromatographic analyses of amino acids inEchinostoma revolutum (Trematoda) adults.Int. J. Parasitol. 7:497–499.
Beaver, P.C. 1937. Experimental studies onEchinostoma revolutum (Froelich), a fluke from birds and mammals.Ill. Biol. Monogr. 15:1–96.
Bennett, S., andFried, B. 1983. Densitometric thin layer chromatographic analyses of free sterols inEchinostoma revolutum (Trematoda) adults and their excretory-secretory products.J. Parasitol. 69:789–790.
Folch, J., Lees, M., andSloane-Stanley, G.H. 1957. A simple method for the isolation and purification of total lipids from animal tissue.J. Biol. Chem. 226:497–509.
Fried, B., andAppel, A.J. 1977. Excretion of lipids byEchinostoma revolutum (Trematoda) adults.J. Parasitol. 63:447.
Fried, B., andButler, M.S. 1977. Histochemical and thin layer chromatographic analyses of neutral lipids in metacercarial and adultCotylurus sp. (Trematoda: Strigeidae).J. Parasitol. 63:831–834.
Fried, B., andGiosca, R.M. 1976. Tentative indication of cholesterol as a chemoattractant for metacercarial pairing inLeucochloridiomorpha constantiae.J. Parasitol. 62:326–327.
Fried, B., andRobinson, G.A. 1981. Pairing and aggregation ofAmblosoma suwaense(Trematoda: Brachylaimidae) metacercariaein vitro and partial characterization of lipids involved in chemoattraction.Parasitology 82:225–229.
Fried, B., andWeaver, L.J. 1969. Exposure of chicks to the metacercaria ofEchinostoma revolutum (Trematoda).Proc. Helminthol. Soc. Wash. 36:153–155.
Fried, B., Tancer, R.B., andFleming, S.J. 1980.In vitro pairing ofEichinostoma revolutum (Trematoda) metacercariae and adults, and characterization of worm products involved in chemoattraction.J. Parasitol. 66:1014–1018.
Little, R.D. 1944. Various oil soluble dyes as fat stains in the super-saturated isopropanol technique.Stain Technol. 19:55–58.
Lutz, P.L., andSiddiqi, A.H. 1971. Nonprotein nitrogenous composition of the protonephridial fluid of the trematodeFasciola gigantica.Comp. Biochem. Physiol. 40A:453–457.
Siddiqi, A.H. 1971. A simple method of collecting urine ofFasciola gigantica for micro-analysis.Ind. J. Helminthol. 23:54–55.
Sonenshine, D.E., Gainsburg, D.M., Rosenthal, M., andSllverstein, R.M. 1981. The sex pheromone glands ofDermacentor variables (Say) andDermacentor andersoni Stiles: Sex pheromone stored in neutral lipid.J. Chem. Ecol. 7:345–357.
von Brand, T. 1973. Biochemistry of Parasites, 2nd ed. Academic Press, New York.
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Gallo, G.J., Fried, B. Association of particular systems with the release of neutral lipids inEchinostoma revolutum (Trematoda) adults. J Chem Ecol 10, 1065–1069 (1984). https://doi.org/10.1007/BF00987513
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DOI: https://doi.org/10.1007/BF00987513