Abstract
We performed field tests of alkaloid induction inNicotiana attenuata plants growing in southwestern Utah with mimicry of the two major types of damage inflicted by invertebrate and vertebrate herbivores: leaf damage and stalk removal, respectively. In undamaged plants, seasonal increases in leaf nicotine content occurred at a rate of 0.046% leaf dry mass/day. Leaf damage doubled the accumulation rate to 0.086–0.138% leaf dry mass/day, while stalk removal resulted in a quadrupling of the accumulation rate to 0.206% leaf dry mass/day. These damage-induced increases in nicotine accumulation are significantly larger than between-plant and phenological variations. Leaf damage to the nornicotine-(N. repanda andN. trigonophylla) and anabasine-accumulating (N. glauca)Nicotiana species native to North America resulted in 1.5- to 5-fold increases in their principal leaf alkaloid pools. We conclude that alkaloid induction is not limited to nicotine-accumulatingNicotiana species and that herbivores feeding on previously damaged plants are likely to encounter tissues with alkaloid titers significantly higher than those of undamaged plants.
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Baldwin, I.T. 1988a. Damaged-induced alkaloids in tobacco: Pot-bound plants are not inducible.J. Chem. Ecol. 4:1113–1120.
Baldwin, I.T. 1988b. Short-term damage-induced increases in tobacco alkaloids protect plants.Oecologia 75:367–370.
Baldwin, I.T. 1989. The mechanism of damaged-induced alkaloids in wild tobacco.J. Chem. Ecol. 15:1661–1680.
Baldwin, I.T. 1991. Damage-induced alkaloids in wild tobacco, pp. 47–69,in D.W. Tallamy and M.J. Raupp (eds.). Phytochemical Induction by Herbivores. John Wiley & Sons, New York.
Baldwin, I.T. 1993. Chemical changes rapidly induced by folivory,in E.A. Bernays (ed.). Insect-Plant Interactions. CRC Press, Boca Raton, Florida. In press.
Baldwin, I.T., andOhnmeiss, T.E. 1993. Coordination of photosynthetic and alkaloidal responses to leaf damage in pot-bound and inducibleNicotiana sylvestris. Submitted.
Baldwin, I.T., Sims, C.L., andKean, S.E. 1990. Reproductive consequences associated with inducible alkaloidal responses in wild tobacco.Ecology 71:252–262.
Bentley, B.L., Johnson, N.D., andRigney, L. 1987. Short-term induction in leaf tissue alkaloids in lupines following experimental defoliation.Am. J. Bot. 74:646.
Coleman, J.S., andJones, C.G. 1991. A phytocentric perspective of phytochemical induction by herbivores, pp. 3–45,in D.W. Tallamy and M.J. Raupp (eds.). Phytochemical Induction by Herbivores. John Wiley & Sons, New York.
Denno, R.F., andMcClure, M.S. 1983. Variable Plants and Herbivores in Natural and Managed Systems. Academic Press, New York.
Duffey, S.S., Bloem, K.A., andCampbell, B.C. 1986. Consequences of sequestration of plant natural products in plant-insect-parasitoid interactions, pp. 31–60,in D.J. Boethel and R.D. Eikenbarry (eds.). Interactions of Plant Resistance and Parasitoids and Predators of Insects. Ellis Horwood, Chicester, England.
Gershenzon, J. 1984. Changes in the levels of plant secondary metabolites under nutrient and water stress.Recent Adv. Phytochem. 18:273–321.
Gutierrez, J.R., andWhitford, W.G. 1987. Chihuahuan desert annuals: Importance of water and nitrogen.Ecology 68:2032–2045.
Johnson, N.D., andBentley, B.L. 1988. Effects of dietary protein and lupine alkaloids on growth and survivorship ofSpodoptera eridania.J. Chem. Ecol. 14:1391–1403.
Johnson, N.D., Rigney, L., andBentley, B.L. 1988. Short-term changes in alkaloid levels following leaf damage in lupines with and without symbiotic nitrogen fixation.J. Chem. Ecol. 15:2425–2434.
Poorter, H., andLewis, C. 1986. Testing differences in relative growth rate: A method avoiding curve fitting and pairing.Physiol. Plant. 67:223–226.
Ralphs, M.H., andWilliams, C. 1988. Alkaloid response to defoliation of velvet lupine (Lupinus leucophyllus).Weed Technol. 2:429–432.
Rosenthal, G.A., andBerenbaum, M.A. 1991. Herbivores, Their Interactions with Secondary Plant Metabolites. Academic Press, New York.
Rossiter, M.C., Schultz, J.C., andBaldwin, I.T. 1988. Relationships among defoliation, red oak phenolics, and gypsy moth growth and reproduction.Ecology 69:267–277.
Saitoh, F., Noma, M., andKawashima, N. 1985. The alkaloid contents of sixtyNicotiana species.Phytochemistry 24:477–480.
Tallamy, D.W., andRaupp, M.J. 1991. Phytochemical Induction by Herbivores. John Wiley & Sons, New York.
van der Meijden, E., van Bemmelen, M.J., Kooi, R., andPost, B.J. 1984. Nutritional quality and chemical defence in the ragwort-cinnabar moth interaction.J. Anim. Ecol. 53:443–453.
Waterman, P.G., andMole, S. 1989. Extrinsic factors influencing production of secondary metab-olites in plants, pp. 107–134,in E.A. Bernays (ed.). Insect-Plant Interactions Boca Raton, Florida.
Wink, M. 1983. Wounding-induced increase of quinolizidine alkaloid accumulation in the lupin leaves.Z. Naturforsch. 38:905–909.
Wink, M. 1985. Chemical defense of lupins: Biological function of quinolizidine alkaloids.Plant Syst. Evol. 150:65–81.
Wink, M. 1987. Chemical ecology of quinolizidine alkaloids, pp. 326–333,in G.R. Waller (ed.). Allelochemicals: Role in Agriculture and Forestry. American Chemical Society, Washington, D.C.
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Baldwin, I.T., Ohnmeiss, T.E. Alkaloidal responses to damage inNicotiana native to North America. J Chem Ecol 19, 1143–1153 (1993). https://doi.org/10.1007/BF00987376
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DOI: https://doi.org/10.1007/BF00987376