Abstract
Tyrosine, tryptophan, and their metabolites in the brain of ddY, non-stimulated El (El (−)), and stimulated El (El (+)) mice were measured using the three dimensional HPLC. The tryptophan content was lower in El (+) than ddY and El (−) mice. The 5-hydroxytrytophan content was much higher in both El groups. The serotonin content of El (+) was higher than that of ddY and El (−) mice. The kynurenine content was remarkably high in the El mice. The dopamine content was lower in El (−) than in ddY mice, whereas it was greater in El (+) than in El (−) mice. The norepinephrine showed higher levels in El (+) mice. These facts suggest that El mice posess congenital metabolic abnormalities of tryptophan and tyrosine and that kynurenine may play an important role as convulsant in El mice seizures along with changes in serotonin, dopamine, and norepinephrine that are inhibitory agents and responded to the repetitive convulsions.
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Imaizumi, K., Ito, S., Kutsukake, G., Takizawa, T., Fujiwara, K., and Tutikawa, K. 1959. Epileptic abnormality of mice. Jikken Dobutsu 8:6–10 (in Japanese).
Imaizumi, K., and Nakano, T., 1964. Mutant stocks, strain, El. Mouse News Letter. 31:57.
Fujiwara, A., Matsumoto, M., and Mori, A. 1977. The content of acetylcholine in the brain of El, CBA and A strain mice. Neurosciences. 3:124–145 (in Japanese).
Fujiwara, M. 1980. Acetylcholine level of mouse brain in preconvulsive states. Neurosciences. 6:192–202.
Kurokawa, M., Machiyama, Y., and Kato, M. 1963. Distribution of acetylcholine in the brain during various states of activity. J. Neurochem. 10:341–348.
Kurokawa, M., Naruse, H., and kato, M. 1966. Metabolic studies on ep mouse, a special strain with convulsive predisposition. Progress in Brain Res. 21A:112–130.
Naruse, H., Kato, M., Kurukawa, M., Haba, R. and Yabe, T. 1960. Metabolic defects in a convulsive strain of mouse. J. Neurochem. 5:359–369.
Hattori, H., Ito, M., and Mikawa, H. 1985. Gamma-aminobutyric acid binding sites and gamma-aminobutyric acid concentrations in epileptic El mouse brain. Eur. J. Pharmacol. 119:217–223.
Hiramatsu, M., Ogawa, K., Kabuto, H., and Mori, A. 1987. Reduced uptake and release of 5-hydroxytryptamine and taurine in the cerebral cortex of epileptic El mice. Epilepsy Res. 1:40–45.
Iwata, H., Yamagami, S., Lee, E., Matsuda, T., and Baba, A. 1979. Increases of brain taurine contents of El mice by physiological stimulation. Jpn. J. Pharmacol. 29:503–507.
Mori, A., Yokoi, I., and Kabuto, H. 1986. 2-Amino-5-phosphonopentanoate prevents convulsions and decreases cerebral glutamate level in El mice. IRCS Med. Sci. 14:475.
Suzuki, S. 1987. Regional changes in amino acid levels in the brain of El mice due to convulsive disposition and seizures. Okayama Igakkai Zasshi. 99:1517–1528 (in Japanese).
Hiramatsu, M. 1983. Brain 5-hydroxytryptamine level, metabolism and binding in El mice. Neurochem. Res. 8:1163–1175.
Hiramatsu, M. 1981. Brain monoamine levels and El mouse convulsions. Folia Psychiatr. Neurol. Jpn. 35:261–266.
Hiramatsu, M., and Mori, A. 1977. Brain catecholamine concentrations and convulsions in El mice. Folia Psychiatr. Neurol. Jpn. 31:491–495.
Hiramatsu, M. and Mori, A. 1980. Distribution of 5-hydroxytryptamine and 5-hydroxyindoleacetic acid in brains of El and ddY mice. IRCS Med. Sci. 8:84.
Hiramatsu, M., and Mori, A. El mouse seizure and 5-hydroxytryptamine in the brain. IRCS Med. Sci. 8:719–720.
Furutsuka, D., Yamagami, S., Chikami, T., Hirayama, E., Mori, K., and Kawakita, Y. 1985. Effect of seizures upon protein synthesis in vitro of synaptosomes from El mouse cerebral cortex. Igakunoayumi. 134:195–196 (in Japanese).
Onishi, H., Yamagami, S., Mori, K., and Kawakita, Y. 1984. Effect of convulsions on the synthesis of heterogeneous nuclear RNA associated with polyadenylate and oligoadenylate sequences from El mouse brain as a convulsive strain. Exp. Neurol. 83:98–107.
Yamagami, S., Onishi, H., Ohno, K., Mori, K., and Kawakita, Y. 1987. Alterations of RNA synthesis in vivo in intact cerebral cortex nuclei induced by convulsions in seizure susceptible El mice. Exp. Neurol. 95:167–177.
Yamagami, S., Ohno, K., Tsuji, M., Mori, K., and Kawakita, Y. 1981. Developmental alteration in RNA metabolism of El mouse brain. Folia Psychiatr. Neurol. Jpn. 35:253–260.
Mody, I., Baimbridge, K. G., and Miller, J. J. 1987. Distribution of calbindin-D28K (CaBP) in the cerebral cortex and hippocampus of the epileptic (El) mouse. Epilepsy Res. 1:46–52.
Mori, A. 1988. El mice: Neurochemical approach to the seizure mechanism. Neuroscience. 14:275–285.
Glowinski, J., and Iverson, L. 1966. Regional studies of catecholamine in the rat brain-I. J. Neurochem. 13:655–669.
Lapin, J. P. 1981. Kynurenines and seizures. Epilepsia. 22:257–265.
Kishikawa, H. 1975. Fundamental and clinical studies of catecholamine metabolism I: fundamental and clinical studies of catecholamine metabolism in epilepsy. Okayama Igakkai Zasshi 87:463–480 (in Japanese).
Mori, A. 1989. Alterations in biogenic amines in epileptic El mouse brain and in human epileptic focus, pages 35–38,in: Suzuki, J., Seino, M., Fukuyama, Y. and Komai, S. (eds.), Art and Science of Epilepsy, Elsevier, Amsterdam.
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Suzuki, S., Mori, A. Regional distribution of tyrosine, tryptophan, and their metabolites in the brain of epileptic El mice. Neurochem Res 17, 693–698 (1992). https://doi.org/10.1007/BF00968007
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DOI: https://doi.org/10.1007/BF00968007