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Localization of leucokinin VIII in the cockroach,Leucophaea maderae, using an antiserum directed against an achetakinin-I analog

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Abstract

An antiserum against an achetakinin analog selectively localized leucokinin VIII (LKVIII) in the CNS ofLeucophaea maderae. Preabsorption studies of the achetakinin antiserum with either preimmune serum or LKVIII prevented a positive reaction in both ELISA and immunocytochemical procedures. LKVIII immunoreactive neurons were found in the brain, frontal, and subesophageal ganglion, all 3 thoracic ganglia and the terminal ganglion. Nerves originating from the thoracic and terminal abdominal ganglia contain LKVIII material. Lateral and medial neurosecretory cells synthesizing LKVIII-like products contribute axons to the nervi corporis cardiaci that terminate in neurohemal sites in the corpora cardiaca and nervi corporis allati. Thus, leucokinin VIII, like leucokinin I (LKI) and leucomyosuppressin (LMS), appears to have both a neurohemal and neurotransmitter mode of regulating target cells inL. maderae.

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References

  1. Holman, G. M., Nachman, R. J., and Wright, M. S. 1990. Insect neuropeptides. Annu. Rev. Entomol. 35:201–217.

    PubMed  Google Scholar 

  2. Hayes, T. K., Pannabecker, T. L., Hinckley, D., Holman, G. M., Nachman, R. J., Pretzel, D., and Beyenback, K. 1989. Leucokinins, a new family of ion transport stimulators and inhibitors in insect Malpighian tubules. Life Sci. 44:1259–1266.

    PubMed  Google Scholar 

  3. Nässel, D. R., Cantera, R., and Karlsson, A. 1992. Neurons in the cockroach nervous system reacting with antisera to the neuropeptide leucokinin I. J. Comp. Neurol. 322:46–67.

    Google Scholar 

  4. Clottens, F. L., Meola, S. M., Coast, G. M., Hayes, T. K., Wright, M. S., Nachman, R. J., and Holman, G. M. 1993. Characterization of an antiserum against an achetakinin I-analog and its use for the localization of culekinin depolarizing peptide II in the mosquito,Culex salinarius. Regul. Pept. 49:145–157.

    PubMed  Google Scholar 

  5. Endo, Y., Iwanaga, T., Fujita, T., and Nishiitsutsuji-Uwo, J. 1982. Localization of pancreatic polypeptide (PP)-like immunoreactivity in the central and visceral nervous systems of the cockroachPeriplaneta. Cell Tiss. Res. 227:1–9.

    Google Scholar 

  6. Weevers, R. deG. 1985. The insect ganglia. Pages 213–297,in Kerkut, G. A., and Gilbert, L. I. (eds.), Comprehensive Insect Physiology, Biochemistry and Pharmacology, Vol. 5, Pergamon Press, Oxford.

    Google Scholar 

  7. Holman, G. M., Cook, B. J., and Nachman, R. J. 1986. Isolation, primary structure and synthesis of two neuropeptides fromLeucophaea maderae: Members of a new family of cephalomyotropins. Comp. Biochem. Physiol. 84C:205–211.

    Google Scholar 

  8. Holman, G. M., Cook, B. J., and Nachman, R. J. 1986. Primary structure and synthesis of two additional neuropeptides fromLeucophaea maderae: Members of a new family of cephalomyotropins. Comp. Biochem. Physiol. 84C:271–276.

    Google Scholar 

  9. Holman, G. M., Cook, B. J., and Nachman, R. J. 1987. Isolation, primary structure and synthesis of leucokinins V and VI: Myotropic peptides ofLeucophaea maderae. Comp. Biochem. Physiol. 88C:27–30.

    Google Scholar 

  10. Holman, G. M., Cook, B. J., and Nachman, R. J. 1987. Isolation, primary structure and synthesis of leucokinins VII and VIII: The final members of this new family of cephalomyotropic peptides from head extracts ofLeucophaea maderae. Comp. Biochem. Physiol. 88C:31–34.

    Google Scholar 

  11. Holman, G. M., Cook, B. J., and Nachman, R. J. 1986. Primary structure and synthesis of a blocked myotropic neuropeptide from the cockroach,Leucophaea maderae. Comp. Biochem. Physiol. 85C:219–224.

    Google Scholar 

  12. Nachman, R. J., Holman, G. M., Haddon, W. F., and Ling, N. 1986. Leucosulfakinin, a sulfated insect neuropeptide with homology to gastrin and cholecystokinin. Science 234:71–73.

    PubMed  Google Scholar 

  13. Nachman, R. J., Holman, G. M., Cook, B. J., Haddon, W. F., and Ling, N. 1986. Leucosulfakinin-II, a blocked sulfated insect neuropeptide with homology to cholecystokinin and gastrin. Biochem. Biophys. Res. Commun. 140:357–364.

    PubMed  Google Scholar 

  14. Holman, G. M., Cook, B. J., and Nachman, R. J. 1986. Isolation, primary structure and synthesis of leucomyosuppressin, an insect neuropeptide that inhibits the spontaneous contractions of the cockroach hindgut. Comp. Biochem. Physiol. 85C:329–333.

    Google Scholar 

  15. Holman, G. M., Nachman, R. J., and Wright, M. S. 1990. A strategy for the isolation and structural characterization of certain insect myotropic peptides that modify the spontaneous contractions of the isolated cockroach hindgut. Pages 195–204,in McCaffery, A. R., and Wilson, I. D. (eds.), Chromatography and Isolation of Insect Hormones and Pheromones, Plenum Press, New York.

    Google Scholar 

  16. Hayes, T. K., Holman, G. M., Pannabecker, T. L., Wright, M. S., Strey, A. A., Nachman, R. J., Hoel, D. F., Olson, J. K., and Beyenbach, K. W. 1993. Culekinin depolarizing peptide: A mosquito leucokinin-like peptide that influences insect Malpighian tubule ion transport. Regul. Pept.,in press.

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Authors and Affiliations

  1. Food Animal Protection Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Route 5, Box 810, 77845, College Station, Texas

    Shirlee M. Meola, Frank L. Clottens & G. Mark Holman

  2. Department of Biology, Birkbeck College, WC1E 7HX, London, England

    Geoffrey M. Coast

Authors
  1. Shirlee M. Meola
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  2. Frank L. Clottens
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  3. Geoffrey M. Coast
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  4. G. Mark Holman
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Meola, S.M., Clottens, F.L., Coast, G.M. et al. Localization of leucokinin VIII in the cockroach,Leucophaea maderae, using an antiserum directed against an achetakinin-I analog. Neurochem Res 19, 805–814 (1994). https://doi.org/10.1007/BF00967448

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