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Uptake ofpara-tyramine andmeta-tyramine into slices of the caudate nucleus and hypothalamus of the rat

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Abstract

The kinetics of the uptake ofp-tyramine,m-tyramine, and dopamine were investigated in slices of the hypothalamus and striatum of the rat in the presence of nialamide. When uptake was analyzed by a least-squares fit to a Lineweaver-Burk plot, each amine appeared to be concentrated by both a “low”-affinity and a “high”-affinity system in both brain regions. The obtainedK m andV max values for the “high”-affinity uptake system for each amine in both brain regions were similar. In general terms, the uptake systems in the striatum exhibited largerK m andV max values, with the velocity of uptake being in the order dopamine>m-tyramine>p-tyramine. 2,4-Dinitrophenol (DNP) and ouabain reduced all uptakes in the caudate, but reduced only the “high”-affinity uptake ofm-tyramine and the “low”-affinity uptake of dopamine in the hypothalamus.

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  1. Elena H. Petrali

    Present address: Department of Physiology, Faculty of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

Authors and Affiliations

  1. Psychiatric Research Division, University Hospital, S7N OXO, Saskatoon, Saskatchewan, Canada

    Elena H. Petrali, Alan A. Boulton & Lillian E. Dyck

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  2. Alan A. Boulton
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  3. Lillian E. Dyck
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Petrali, E.H., Boulton, A.A. & Dyck, L.E. Uptake ofpara-tyramine andmeta-tyramine into slices of the caudate nucleus and hypothalamus of the rat. Neurochem Res 4, 633–642 (1979). https://doi.org/10.1007/BF00964440

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