Abstract
Detailed information is presented on the changes in lipid-class profiles (polar lipids, total neutral lipids, free sterols, steryl esters, triacylglycerols, neutral ether lipids and free fatty acids), which are manifest during the annual reproductive cycle of the seastarAsterias rubens for both the storage organs (pyloric caeca) and utilizing organs (ovaries). The ovaries appear to accumulate all individual lipid components proportional to progression of vitellogenesis. Pyloric caeca, in contrast, show variable lipid-class levels, probably due to more complex processes in these organs, operating simultaneously: digestion of food, accumulation of storage lipids, mobilization of stored lipids and their release. The results are discussed in terms of current knowledge on the role of lipids in echinoderm reproduction.
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Literature Cited
Ackman, R.G., C.A. Eaton, J. Kinneman and C. Litchfield: Lipids of freshwater dolphinSotalia fluviatilis: comparison of odontocete bioacoustic lipids and habitat. Lipids10, 44–49 (1975)
Allen, W.V.: Fatty-acid synthesis in the echinoderms:Asterias rubens, Echinus esculentus andHolothuria forskali. J. mar. biol. Ass. U.K.48, 521–533 (1968)
—: Biochemical aspects of lipid storage and utilization in animals. Am. Zool.16, 631–647 (1976)
— and A.C. Giese: Anin vitro study of lipogenesis in the sea star,Pisaster ochraceus. Comp. Biochem. Physiol.17, 23–38 (1966)
Bergelson, L.D.: Diol lipids. Prog. Chem. Fats10, 239–286 (1969)
Björkman, L.R., K.-A. Karlsson and K. Nilsson: On the existence of cerebroside and cholesterol sulfate in tissues of the sea starAsterias rubens. Comp. Biochem. Physiol.43B, 409–411 (1972a)
——, I. Pascher and B.E. Samuelsson: The identification of large amounts of cerebroside and cholesterol sulfate in the sea star,Asterias rubens. Biochim. biophys. Acta270, 260–265 (1972b)
Bligh, E.G. and W.J. Dyer: A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol.37, 911–917 (1959)
Boolootian, R.A.: Reproductive physiology.In: Physiology of Echinodermata, pp 561–613. Ed. by R.A. Boolootian. New York: Interscience Publishers 1966
Downing, D.T.: Photodensitometry in the thinlayer chromatographic analysis of neutral lipids. J. Chromat.38, 91–99 (1968)
Ferguson, J.C.: The annual cycle of fatty acid composition in a starfish. Comp. Biochem. Physiol.54B, 249–252 (1976)
Fewster, M.E., B.J. Burns and J.F. Mead: Quantitative densitometric thin-layer chromatography of lipids using copper acetate reagent. J. Chromat.43, 120–126 (1969)
Freeman, C.P. and D. West: Complete separation of lipid classes on a single thin-layer plate. J. Lipid Res.7, 324–327 (1966)
Giese, A.C.: On the biochemical constitution of some echinoderms.In: Physiology of Echinodermata, pp 757–796. Ed. by R.A. Boolootian. New York: Interscience Publishers 1966
Lawrence, J.M.: Level, content, and caloric equivalents of the lipid, carbohydrate, and protein in the body components ofLuidia clathrata (Echinodermata: Asteroidea: Platyasterida) in Tampa Bay. J. exp. mar. Biol. Ecol.11, 263–274 (1973)
—: Patterns of lipid storage in post-metamorphic marine invertebrates. Am. Zool.16, 747–762 (1976)
Lee, R.F., J.C. Nevenzel and G.-A. Paffenhöfer: Importance of wax esters and other lipids in the marine food chain: phytoplankton and copepods. Mar. Biol.9, 99–108 (1971)
—, C.F. Phleger and M.H. Horn: Composition of oil in fish bones: function in neutral buoyancy. Comp. Biochem. Physiol.50B, 13–16 (1975)
Mackie, A.M., H.T. Singh and J.M. Owen: Studies on the distribution, biosynthesis and function of steroidal saponins in echinoderms. Comp. Biochem. Physiol.56B, 9–14 (1977)
Nevenzel, J.C.: Occurrence, function and biosynthesis of wax esters in marine organisms. Lipids5, 308–319 (1970)
Oudejans, R.C.H.M. and D.J. van der Horst: Effect of excessive fatty acid ingestion upon composition of neutral lipids and phospholipids of snailHelix pomatia L. Lipids9, 798–803 (1974)
— and I. van der Sluis: Changes in the biochemical composition of the ovaries of the seastarAsterias rubens during its annual reproductive cycle. Mar. Biol.50, 255–261 (1979)
—— and A.J. van der Plas: Changes in the biochemical composition of the pyloric caeca of female seastars,Asterias rubens, during their annual reproductive cycle. Mar. Biol.53, 231–238 (1979)
Rodegker, W. and J.C. Nevenzel: The fatty acid composition of three marine invertebrates. Comp. Biochem. Physiol.11, 53–60 (1964)
Sargent, J.R.: The structure, metabolism and function of lipids in marine organisms. Biochem. biophys. Perspect. mar. Biol.3, 149–212 (1976)
Schoenmakers, H.J.N.:In vitro biosynthesis of steroids from cholesterol by the ovaries and pyloric caeca of the starfishAsterias rubens. Comp. Biochem. Physiol. (In press). (1979)
Snyder, F., B. Malone and M.L. Blank: The biosynthesis of alkyl glyceryl ethers by microsomal enzymes of digestive glands and gonads of the starfish,Asterias forbesi. Biochim. biophys. Acta187, 302–306 (1969)
Vaver, V.A., N.A. Pisareva and L.D. Bergelson: Diol lipids. XXI: The high ethyleneglycol content of marine invertebrate lipids. Chemy Phys. Lipids8, 82–86 (1972)
——, B.V. Rozynov, A.N. Ushakov and L.D. Bergelson: Diol lipids, XX. Alkyl and alk-1-enyl ethers of ethanediol in lipids of starfish. Chemy Phys. Lipids7, 75–92 (1971)
Vinson, J.A. and J.E. Hooyman: Sensitive fluorogenic visualization reagent for the detection of lipids on thin-layer chromatograms. J. Chromat.135, 226–228 (1977)
Voogt, P.A. and J.W.A. van Rheenen: On the fate of dietary sterols in the sea starAsterias rubens. Comp. Biochem. Physiol.54B, 473–477
——: On the origin of the sterols in the sea starAsterias rubens. Comp. Biochem. Physiol.54B, 479–482 (1976b)
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Communicated by O. Kinne, Hamburg
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Oudejans, R.C.H.M., van der Sluis, I. Storage and depletion of lipid components in the pyloric caeca and ovaries of the seastarAsterias rubens during its annual reproductive cycle. Mar. Biol. 53, 239–247 (1979). https://doi.org/10.1007/BF00952432
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DOI: https://doi.org/10.1007/BF00952432