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Detection of loss of heterozygosity in theAPC tumor suppressor gene in nonpapillary renal cell carcinoma by microdissection and polymerase chain reaction

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Abstract

The role of theAPC (adenomatouspolyposiscoli) tumor suppressor gene in the genesis of nonpapillary renal cell carcinoma is addressed. The frequency of allelic deletion in theAPC gene was analyzed using microdissection of the tumor specimens and a PCR (polymerase chain reaction)-based assay for the detection of intragenic loss of heterozygosity (LOH). Twelve of 29 carcinomas investigated were informative (41%). In five of these (42%) LOH was detected in theAPC gene. LOH did not correlate with tumor grade or stage. This high frequency of intragenic LOH suggests an implication of theAPC gene or a closely linked gene in the genesis of a subset of nonpapillary renal cell carcinoma. The use of a microdissection technique allows the reliable detection of tumor-specific LOH when using a PCR-based assay.

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Authors and Affiliations

  1. Urologische Klinik und Poliklinik, Universitätsklinikum, D-45122, Essen, Germany

    M. Böhm, C. Stinhöfer, T. Otto & H. Rübben

  2. Institut für Zellbiologie (Tumorforschung), Universitätsklinikum Essen, D-45122, Essen, Germany

    I. Wieland & C. Stinhöfer

Authors
  1. M. Böhm
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  2. I. Wieland
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  3. C. Stinhöfer
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  4. T. Otto
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  5. H. Rübben
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Böhm, M., Wieland, I., Stinhöfer, C. et al. Detection of loss of heterozygosity in theAPC tumor suppressor gene in nonpapillary renal cell carcinoma by microdissection and polymerase chain reaction. Urol. Res. 25, 161–165 (1997). https://doi.org/10.1007/BF00941976

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  • DOI: https://doi.org/10.1007/BF00941976

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