Abstract
Nucleotide sequence variations in a region of the mitochondrial cytochromec oxidase subunit I (COI) gene (391 bp) were examined within seven species of the genusTaenia and two species of the genusEchinococcus, including ten isolates ofT. taeniaeformis and six isolates ofE. multilocularis. More than a 12% rate of nucleotide differences between taeniid species was found, allowing the species to be distinguished. InE. multilocularis, no sequence variation was observed among isolates, regardless of the host (gray red-backed vole, tundra vole, pig, Norway rat) or area (Japan, Alaska) from which each metacestode had been isolated. In contrast, six distinct sequences were detected among the tenT. taeniaeformis isolates examined. The level of nucleotide variation in the COI gene withinT. taeniaeformis isolates except for one isolate from the gray red-backed vole (TtACR), which has been proposed as a distinct strain or a different species, was about 0.3%–4.1%, whereas the COI gene sequence for TtACR differed from those of the other isolates, with levels being 9.0%–9.5%. Phylogenetic trees were then inferred from these sequence data using two different algorithms.
Similar content being viewed by others
References
Abuladze KI (1964) Taeniata of animals and man, and diseases caused by them. Akademiya Nauk USSR. Moscow, pp 59–67 (translated by Israel's Program for Scientific Translation, Jerusalem, 1970)
Azuma H, Okamoto M, Oku Y, Kamiya M (1995) Intraspecific variation ofTaenia taeniaeformis as determined by various criteria. Parasitol Res 81:103–108
Bessho Y, Ohama T, Osawa S (1992a) Planarian mitochondria. I. Heterogeneity of cytochrome c oxidase subunit I gene sequences in the freshwater planarian,Dugesia japonica. J Mol Evol 34:324–330
Bessho Y, Ohama T, Osawa S (1992b) Planarian mitochondria. II. The unique genetic code as deduced from cytochrome c oxidase subunit I gene sequences. J Mol Evol 34:331–335
Bowles J, McManus DP (1993) Molecular variation inEchinococcus. Acta Trop (Basel) 53:291–305
Bowles J, Blair D, McManus DP (1992) Genetic variants within the genusEchinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol 54:165–174
Crozier RH, Crozier YC, Mackinlay AG (1989) The CO-I and CO-II region of honeybee mitochondrial DNA: evidence for variation in insect mitochondrial evolutionary rates. Mol Biol Evol 6:399–411
Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376
Felsenstein J (1989) PHYLIP: phylogeny inference package (version 3.2). Cladistics 5:164–166
Felsenstein J (1993) PHYLIP, version 3.5, 1st edn. University of Washington Press, Seattle
Higgins DG, Bleasby AJ, Fuchs R (1992) CLUSTAL V: improved software for multiple sequence alignment. CABIOS 8:189–191
Iwaki T, Nonaka N, Okamoto M, Oku Y, Kamiya M (1994) Developmental and morphological characteristics ofTaenia taeniaeformis (Batsch, 1786) inClethrionomys rufocanus bedfordiae andRattus norvegicus from different geographical locations. J Parasitol 80:461–467
Lecanidou R, Douris V, Rodakis GC (1994) Novel features of metazoan mtDNA revealed from sequence analysis of three mitochondrial DNA segments of the land snailAlbinaria turrita (Gastropoda: Clausiliidae). J Mol Evol 38:369–382
Lewis JW, Twigg GI (1972) A study of the internal parasites of small rodents from woodland areas in Surrey. J Zool 166:61–77
McManus DP, Bowles J (1994) Asian (Taiwan)Taenia: species or strain? Parasitol Today 10:273–275
Miyauchi T, Sakui M, Ishige M, Fukumoto S, Ueda A, Ito M, Ohbayashi M (1984) A case of multilocular echinococcosis in a horse. Jpn J Parasitol 32:171–173
Moore J (1981) Asexual reproduction and environmental predictability in cestodes (Cyclophyllidea: Taeniidae). Evolution 35:723–741
Moore J, Brooks D (1987) Asexual reproduction in cestodes (Cyclophyllidea: Taeniidae): ecological and phylogenetic influences. Evolution 41:882–891
Murai E, Tenora F (1973) Some taeniid species (Cestoidea) parasitizing vertebrates (Rodentia, Carnivora, Strigiformes) in Hungary. Acta Zool Acad Sci Hung 19:125–132
Nonaka N, Iwaki T, Okamoto M, Ooi H-K, Oku Y, Ohbayashi M, Kamiya M (1994) Infectivities of four isolates ofTaenia taeniaeformis to various rodents. J Vet Med Sci 56:565–567
Okamoto M, Fujita O, Arikawa J, Kurosawa T, Oku Y, Kamiya M (1992) NaturalEchinococcus multilocularis infection in a Norway rat,Rattus norvegicus, in southern Hokkaido, Japan. Int J Parasitol 22:681–684
Okamoto M, Ito A, Kurosawa T, Oku Y, Kamiya M, Agatsuma T (1995) Intraspecific variation of isoenzymes inTaenia taeniaeformis. Int J Parasitol 25:221–228
Rupes V (1964) Parasitic worms ofApodemus flavicollis andClethrionomys glareolus from the environs of Prague. Cesk Parasitol 11:335–338
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Sakui M, Ishige M, Fukumoto S, Ueda A, Ohbayashi M (1984) SpontaneousEchinococcus multilocularis infection in swine in north-eastern Hokkaido, Japan. Jpn J Parasitol 33:291–296
Schmidt GD (1986) CRC handbook of tapeworm identification. CRC, Boca Raton, Florida
Yamashita J (1973)Echinococcus and echinococcosis. In: Morishita K, Komiya Y, Matsubayashi H (eds) Progress of medical parasitology in Japan, vol 5. Megro Parasitological Museum, Tokyo, pp 65–123
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Okamoto, M., Bessho, Y., Kamiya, M. et al. Phylogenetic relationships withinTaenia taeniaeformis variants and other taeniid cestodes inferred from the nucleotide sequence of the cytochromec oxidase subunit I gene. Parasitol Res 81, 451–458 (1995). https://doi.org/10.1007/BF00931785
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00931785