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Cyclic nucleotide changes induced in human leukocytes by a product of axenically grownEntamoeba histolytica that inhibits human monocyte locomotion

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Abstract

Pulse exposure of human mononuclear phagocytes to the monocyte locomotion-inhibitory factor produced byEntamoeba histolytica (i.e., the 369- to 765-Da chromatographic fraction obtained from the supernatant fluid of axenically grownE. histolytica) led to a swift increase in the intracellular concentration of adenosine 3′:5′ cyclic monophosphate (cAMP). A weaker response was observed in human polymorphonuclear leukocytes, the locomotion of which, however, is not inhibited by this amebic factor. The same chromatographic fraction obtained from the axenic medium control lacked this effect, at least upon mononuclear phagocytes. On the other hand, both the monocyte locomotion-inhibitory factor and the axenic medium control, possibly through shared culture medium components, induced comparable increases in guanosine 3′:5′ cyclic monophosphate (cGMP) in human mononuclear phagocytes and in polymorphonuclear leukocytes, thus suggesting that the latter nucleotide is not critical for the leukotactic inhibitory phenomenon. Our results suggest that like other leukotactic inhibitors, the monocyte locomotion-inhibitory factor produced byE. histolytica operates through modulations of intracellular cAMP.

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Abbreviations

MP :

Mononuclear phagocytes (monocytes)

MLIF :

monocyte locomotion-inhibitory factor

PMN :

polymorphonuclear leukocytes

cAMP :

adenosine 3′:5′ cyclic monophosphate

cGMP :

guanosine 3′:5′ cyclic monophosphate

PBS :

phosphate-buffered (pH 7.4) normal saline

AMC :

axenic medium control Gey's-A; Gey's medium with 2% albumin

ZAS :

zymosan-activated serum

hpf :

high-power field (400x)

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Authors and Affiliations

  1. Unidad de Immunología, Subjefatura de Investigación Biomédica, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social and Subdivisión de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Apto. Postal 73-032, CP 03020, México, D.F., México

    G. Rico, O. Díaz-Guerra & R. R. Kretschmer

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  1. G. Rico
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  2. O. Díaz-Guerra
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  3. R. R. Kretschmer
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Rico, G., Díaz-Guerra, O. & Kretschmer, R.R. Cyclic nucleotide changes induced in human leukocytes by a product of axenically grownEntamoeba histolytica that inhibits human monocyte locomotion. Parasitol Res 81, 158–162 (1995). https://doi.org/10.1007/BF00931623

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  • DOI: https://doi.org/10.1007/BF00931623

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