Abstract
A blood-meal-induced lectin (agglutinin) with proteolytic activity was isolated from midgut extracts ofGlossina longipennis by a two-step procedure involving anion-exchange chromatography. It is a glycoprotein [native molecular weight (Mr, 61000±3000 da) composed of two noncovalently-linked subunits designated α (Mr, ∼27000 da) and β (Mr, ∼33000 da). The trypsin activity and the glycosyl residues were present on the α- and β-subunits, respectively. The native protein was capable of agglutinating both bloodstream-form and procyclic trypanosomes as well as rabbit red blood cells. This activity was strongly inhibited byD-glucosamine and weakly inhibited byN-acetyl-D-glucosamine. Similarly, soybean trypsin inhibitor abrogated agglutination of bloodstream-form parasites, whereas the procyclics were unaffected. The agglutination activity was sensitive to temperatures above 40° C but was unaffected by chelators of metal ions. Antibodies raised against the protein were used in immunoblotting experiments to show the presence of a similar protein in several members of theGlossina species. However, no cross-reactivity was detected with midgut extracts prepared from sandflies, mosquitoes, or stable flies. It is proposed that this molecule might play an important role in differentiation of bloodstream-form trypanosomes into procyclic (midgut) forms.
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Osir, E.O., Abubakar, L. & Imbuga, M.O. Purification and characterization of a midgut lectin-trypsin complex from the tsetse flyGlossina longipennis . Parasitol Res 81, 276–281 (1995). https://doi.org/10.1007/BF00931530
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DOI: https://doi.org/10.1007/BF00931530