Summary
Extracellular thermostable neutral proteinase was produced byBacillus stearothermophilus strains NCIB 8924 and NRRL B-3880 growing at 55°C. The formation and stabilization of this proteinase was found to be dependent on the concentration of free calcium ions. Therefore, procedures that removed free calcium ions from the medium, such as the use of phosphate buffer, resulted in a lower production of proteinase. The calcium-deficient proteinase was denaturated or adsorbed by calcium phosphate compounds. During the sterilization procedure of the culture medium, the CaCO3 precipitation, caused by the removal of CO2, influenced the amount of proteinase produced in a phosphate buffered medium made with tap water. An improved medium without phosphate buffer was used for 10 and 300 l batch cultivations and the calcium requirement for proteinase formation by the two strains was determined.
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Sidler, W., Zuber, H. The production of extracellular thermostable neutral proteinase and α-amylase byBacillus stearothermophilus . European J. Appl Microbiol. 4, 255–266 (1977). https://doi.org/10.1007/BF00931262
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DOI: https://doi.org/10.1007/BF00931262