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Differential response of the epidermal growth factor receptor tyrosine kinase activity to several plant and mammalian lectins

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Abstract

Biosignalling via lectins may involve modulation of protein kinase activities. This aspect of the biological action of mammalian and plant lectins has been investigated for their effect on the activity of the isolated epidermal growth factor receptor (EGFR). The constitutive tyrosine kinase activity of the epidermal growth factor receptor from rat liver, isolated by calmodulin-affinity chromatography, was activated by concanavalin A (ConA), and wheat germ agglutinin (WGA) to a similar extent as the measured enhancement induced by EGF. In contrast, two mannose-specific lectins, the mannan-binding protein (MBP) and serum amyloid P component (SAP), isolated from human serum, have inhibitory effects, both in the absence and presence of EGF. The differential effects of these lectins were tested using as phosphorylatable substrates a co-polymer of glutamic acid-tyrosine, as well as calmodulin. However, two galactoside-specific lectins, the laminin-binding β-galactoside-binding 14 kDa lectin, isolated from bovine heart (14K-BHL), and the α/β-galactoside-binding lectin, isolated from mistletoe (Viscum album L.) leaves (VAA), do not inhibit the EGFR tyrosine kinase activity. The sugar dependence of the lectin-mediated action was studied by inhibition assays. Mannose and a mannose-containing neoglycoprotein prevent the activating effect of ConA, and N-acetyl-D-glucosamine partially prevents the activation produced by WGA. However, mannose and mannose-containing neoglycoprotein were ineffective to reduce the inhibitory effect of MBP or SAP. Although the response to binding of ConA and WGA was different to that of MBP or SAP with respect to the tyrosine kinase activity of the EGFR, it should be noted that the four lectins inhibited the binding of [125I]EGF to its receptor with similar efficiency.

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Abbreviations

EGF:

epidermal growth factor

EGFR:

epidermal growth factor receptor

ConA:

concanavalin A

MBP:

mannan-binding protein

SAP:

serum amyloid P component

WGA:

wheat germ agglutinin

14K-BHL:

bovine heart 14 kDa lectin

VAA:

Viscum album L. (mistletoe) agglutinin

EGTA:

[ethylenebis(oxyethylenenitrilo)]-tetraacetic acid; poly(Glu:Tyr)-co-polymer of L-glutamic acid and L-tyrosine

Hepes:

4-(2-hydroxyethyl)-1-piperazinethanesulfonic acid

Tris:

tris(hydroxymethyl)-aminomethane

DSS:

suberic acid bis(N-hydroxy-succinimide ester)

PMSF:

phenylmethanesulfonyl fluoride

Man:

mannose

Gal:

galactose

BSA:

bovine serum albumin

Man-BSA:

neoglycoprotein containing α-D-mannose

Lac-BSA:

neoglycoprotein containing β-lactose

Gal-BSA:

neoglycoprotein containing galactose

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Authors and Affiliations

  1. Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas, Arturo Duperier 4, 28029, Madrid, Spain

    Alberto Benguría, Sabine Kafert & Antonio Villalobo

  2. Institut für Physiologische Chemie der Tierärztlichen Fakultät, Ludwig-Maximilians-Universität, Veterinärstrasse 13, 80539, München, Germany

    Fu-Yue Zeng, Sabine André & Hans-J. Gabius

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  1. Fu-Yue Zeng
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  2. Alberto Benguría
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  4. Sabine André
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  5. Hans-J. Gabius
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  6. Antonio Villalobo
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Zeng, FY., Benguría, A., Kafert, S. et al. Differential response of the epidermal growth factor receptor tyrosine kinase activity to several plant and mammalian lectins. Mol Cell Biochem 142, 117–124 (1995). https://doi.org/10.1007/BF00928932

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