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Hexose metabolism in pancreatic islets: Effect of D-glucose on the mitochondrial redox state

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Abstract

The mitochondrial NADH/NAD+ ratio for free nucleotides in rat pancreatic islets was judged from the cell content in L-glutamate and L-alanine, 2-ketoglutarate and pyruvate, and NH +4 . At a physiological concentration of D-glucose, such a ratio averaged 9.6±1.1%. A rise in hexose concentrations, above a threshold value in excess of 5.6 mM, caused a rapid, sustained and rapidly reversible decrease in the mitochondrial NADH/NAD+ ratio. It is speculated that in the process of glucose-stimulated insulin release, the latter change participates in the coupling between metabolic and secretory events by favouring both the activity of key mitochondrial dehydrogenases and the translocation of Ca2+ from the mitochondria into the cytosol.

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Authors and Affiliations

  1. Laboratory of Experimental Medicine, Erasmus Medical School, Brussels Free University, 808 Route de Lennik, B-1070, Brussels, Belgium

    Remedios Ramirez, Abdullah Sener & Willy J. Malaisse

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  1. Remedios Ramirez
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  2. Abdullah Sener
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  3. Willy J. Malaisse
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Ramirez, R., Sener, A. & Malaisse, W.J. Hexose metabolism in pancreatic islets: Effect of D-glucose on the mitochondrial redox state. Mol Cell Biochem 142, 43–48 (1995). https://doi.org/10.1007/BF00928912

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  • DOI: https://doi.org/10.1007/BF00928912

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