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Light and electron microscopic studies on the effect of praziquantel onSchistosoma mansoni, Dicrocoelium dendriticum, andFasciola hepatica (trematoda) in vitro

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Abstract

The fine structure of the tegument of three trematode species,Schistosoma mansoni, Dicrocoelium dendriticum, andFasciola hepatica, was studied by means of light scanning (SEM) and transmission electron microscopy (TEM) after in vitro exposure to 0, 1,10, and 100 μg/ml of the anthelmintic praziquantel for 5, 15, 30, and 60 min. InS. mansoni andD. dendriticum the resulting vacuolization of the tegument was confined to numerous small areas scattered all over the surface of the parasites and this finally led to the disruption of the apical tegumental layer. No changes were found in the tegument ofF. hepatica after treatment with praziquantel.

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Abbreviations

B:

Bubbles

BL:

Basement layer

CM:

Circular musculature

GA:

Genital atrium

GC:

Gynaecophoric canal

IB:

Inclusion bodies

IM:

Interstitial material

LM:

Longitudinal musculature

MI:

Mitochondria

OS:

Oral sucker

R:

Ridges

S:

Spine

SP:

Surface pits

T:

Tubercle

TG:

Tegument

V:

Vacuoles

VS:

Ventral sucker

SEM:

Scanning electron microscopy

TEM:

Transmission electron microscopy

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Authors and Affiliations

  1. Institut für Zoologie II der Universität Düsseldorf, Universitätsstr. 1, D-4000, Düsseldorf 1, Germany

    B. Becker & H. Mehlhorn

  2. Bayer Pharma-Forschungszentrum, Institut für Chemotherapie, D-5600, Wuppertal 1, Germany

    P. Andrews & H. Thomas

  3. Institut für Parasitologie der Universität Zürich, CH-8057, Zürich, Switzerland

    J. Eckert

Authors
  1. B. Becker
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  2. H. Mehlhorn
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  3. P. Andrews
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  4. H. Thomas
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  5. J. Eckert
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Dedicated to Professor G. Piekarski on the occasion of his 70th birthday

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Becker, B., Mehlhorn, H., Andrews, P. et al. Light and electron microscopic studies on the effect of praziquantel onSchistosoma mansoni, Dicrocoelium dendriticum, andFasciola hepatica (trematoda) in vitro. Z. Parasitenkd. 63, 113–128 (1980). https://doi.org/10.1007/BF00927527

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  • DOI: https://doi.org/10.1007/BF00927527

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