Abstract
Leishmania species grown in culture excrete a polyanionic, carbohydrate-rich factor (EF) which binds to antibodies produced in rabbits against the parentLeishmania species. EF, previously purified by physical and chemical methods, was purified by affinity chromatography on aRicinus lectin column. The purified samples were characterised and analysed. The results show a notable proportion of galactose in EF and clarify the reasons for its polyanionic properties. Heterogenicity of EF is demonstrated and discussed.
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Zehavi, U., Abrahams, J.C., Granoth, R. et al. Leishmanial excreted factors (EFs): Purification by affinity chromatography. Z. Parasitenkd. 69, 695–701 (1983). https://doi.org/10.1007/BF00927419
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DOI: https://doi.org/10.1007/BF00927419