Abstract
Human Tμ, Tγ, and Tφ lymphocyte subpopulations have the capacity to respond to phytohemagglutinin (PHA)in vitro with proliferation and the production of a pH 2 and heat-labile γ interferon. This occurs both when the subsets are isolated by direct rosetting techniques or by negative selection. Macrophages enhance the production of the γ interferon by each lymphocyte subset and do not themselves produce γ interferon in response to products of PHA-activated lymphocyte subsets. Thus our studies indicate that subpopulations of T lymphocytes known to differ with regard to morphology, surface receptors, RNA content, response to corticosteroids and X-irradiation, and other functional capabilities do not differ with regard to their capacity to produce γ interferon.
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Epstein LB, Cline MJ, Merigan TC: The interaction of human macrophages and lymphocytes in the PHA stimulated production of interferon. J Clin Invest 50:744–753, 1971
Epstein LB, Cline MJ, Merigan TC: PPD stimulated interferon:In vitro macrophage lymphocyte interaction in the production of a mediator of cellular immunity. Cell Immunol 2:602–613, 1971
Epstein LB, Stevens DA, Merigan TC: Selective increase in lymphocyte interferon response to vaccinia antigen following revaccination. Proc Nat Acad Sci USA 69:2632–2636, 1972
Epstein LB, Kreth HW, Herzenberg LA: Fluorescence activated cell sorting of human T and B lymphocytes. II. Identification of the cell type responsible for interferon production and cell proliferation in response to mitogens. Cell Immunol 12:407–421, 1974
Gupta S: Functionally distinct subpopulations of human T lymphocytes. A review. Clin Bull 8:100–107, 1978
Gupta S, Fernandes G, Nair M, Good RA: Spontaneous and antibody dependent cell-mediated cytotoxicity by human T cell subpopulations. Proc Nat Acad Sci USA 75:5137–5141, 1978
Goldblatt D, McManus NH, Epstein LB: A micromethod for preparation of human macrophage cultures for the study of lymphocyte-macrophage interaction in immune interferon production and blastogenesis. Immunopharm 1:13–20, 1978
Boyum A: Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest 21 (Suppl 97):77–89, 1968
Gupta S, Good RA: Subpopulations of human T lymphocytes II. Effect of thymopoietin, corticosteroids, and irradiation. Cell Immunol 34:10–18, 1977
Gupta S, Good RA: Subpopulations of human T lymphocytes I. Studies in immunodeficient patients. Clin Exp Immunol 30:222–228, 1977
Frei J, Borel C, Horvath G, Cullity B., Vannotti A: Enzymatic studies in the different types of normal and leukemic human white cells. Blood 18:317–327, 1961
Epstein LB, McManus NH: Macro- and microassays for the antiviral effects of human and mouse interferons.In Manual of Clinical Immunology, 2nd ed, N Rose, H Friedman (eds). Washington, DC, Amer Soc Microbiol, 1980, pp 275–283
Epstein, LB: The comparative biology of immune and classical interferons.In Biology of the Lymphokines, S Cohen, E Pick, JJ Oppenheim (eds). New York, Academic Press, 1979, pp 443–514
Moretta L, Ferrarini M, Mingari MC, Moretta A, Webb SR: Subpopulations of human T cells identified by receptors for immunoglobulins and mitogen responsiveness. J Immunol 117:2171–2174, 1976
Grossi CE, Webb SR, Zicca A, Lydyard PM, Moretta L, Mingari MC, Cooper MD: Morphological and histochemical analysis of two human T cell subpopulations bearing receptors for IgM and IgG. J Exp Med 147:1405–1417, 1978
Andreeff M, Beck, JD, Darzynkiewicz Z, Traganos F, Gupta S, Melamed MR, Good RA: RNA content in human lymphocyte subpopulations. Proc Nat Acad Sci USA 75:1938–1942, 1978
Perussia B, Mangoni L, Engers HD, Trinchieri G: Interferon production by human and murine lymphocytes in response to alloantigens. J Immunol 125:1589–1595, 1980
Kapadia A, O'Reilly RJ, Good RA, Gupta S: Leukocyte migration inhibition factor (LMIF) production by human T cell subpopulation. Fed Proc 37:1365, 1978
Eardley DD, Shen FW, Gershon RK, Ruddle NH: Lymphotoxin production by subsets of T cells. J Immunol 124:1199–1202, 1980
Gupta S, Fernandes G, Rocklin R, Good RA: Histamine receptors on human T cell subsets.In New Trends in Human Immunology and Cancer Immunotherapy, B Serrou, C Rosenfeld (eds). Paris, Dion Editeur, 1980, pp 434–438
Hayward AR, Layward L, Lydyard PM, Moretta L, Dagg M, Lawton AR: Fc receptor heterogeneity of human suppressor T cells. J Immunol 121:1–5, 1978
Pichler WJ, Lum L, Broder S: Fc receptors on human T lymphocytes. I. Transition of Tγ to Tε cells. J Immunol 121:1540–1548, 1978
Schulof RS, Fernandes G, Good RA, Gupta S: Neuraminidase treatment of T lymphocytes. Effect on Fc Receptor phenotype and function. Clin Exp Immunol 40:611–619, 1980
Reinherz, EL, Moretta L, Roper M, Breard JM, Mingari MC, Cooper MD, Schlossman SF: Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison. J Exp Med 151:969–974, 1980
Gupta S, Good RA: Subpopulations of human T lymphocytes: Laboratory and clinical studies. Immunol Rev 56:89–113, 1981
Kaszubowski PA, Husby G, Tung KSK, Williams RC Jr: T lymphocyte subpopulations in peripheral blood and tissues of cancer patients. Cancer Res 40:4648–4657, 1980
Epstein LB: The effects of interferons on the immune response in vitro and in vivo.In Interferons and Their Actions, WE Stewart II (ed). Cleveland, CRC Press, 1977, pp 92–132
Epstein LB: Mitogen and antigen induction of interferon in vitro and in vivo. Tex Rep Biol Med 35:42–56, 1977
Archer DL, Smith BG, Ulrich JT, Johnson HM: Immune interferon induction by T cell mitogens involves different T cell subpopulations. Cell Immunol 48:420–426, 1979
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Epstein, L.B., Gupta, S. Human T-lymphocyte subset production of immune (γ) interferon. J Clin Immunol 1, 186–194 (1981). https://doi.org/10.1007/BF00922762
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DOI: https://doi.org/10.1007/BF00922762