Abstract
Membrane depolarization is an early event in cell stimulation. Since the resting membrane potential is dependent on the potassium composition of the extracellular medium, we investigated whether there are clinical situations in which potassium levels are high enough to depolarize polymorphonuclear leukocytes. We determined the ionic composition of sterile and infected interstitial fluid in humans and guinea pigs. All sterile extravascular fluids had physiological potassium levels in the same range as serum values. In contrast, human abscess fluids contained increased K+-levels (17±6.4 mmol/liter,N=8) and 15 of 20 experimental abscesses in guinea pigs contained>10 mmol/liter K+. In humans three of eight abscess fluid K+ levels and in guinea pigs three of five abscess fluid K+ levels were even>15 mmol/liter. Thus, high K+ levels, previously shown to activate polymorphonuclear leukocytes, are observed in certain clinical situations associated with local inflammation.
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Davis, J. M., andJ. I. Gallin. 1981. The neutrophil.In Cellular Functions in Immunity and Inflammation. J. J. Oppenheim, D. L. Rosenstreich, and M. Potter, editors. Eisevier, New York. 77–102.
Seligmann, B., andJ. I. Gallin. 1983. Abnormality in elicited membrane potential changes in neutrophils from patients with chronic granulomatous disease.In Advances in Host Defense Mechanisms, Vol. 3. J. I. Gallin and A. S. Fauci, editors. Raven Press, New York, 195–226.
Seligmann, B. E., andJ. I. Gallin. 1980. Use of lipophilic probes of membrane potential to assess human neutrophil activation.J. Clin. Invest. 66:483–503.
Showell, H. J., P. H. Naccache, R. I. Sha'afi, andE. L. Becker. 1977. The effects of extracellular K+, Na+, and Ca++ on lysosomal enzyme secretion from polymorphonuclear leukocytes.J. Immunol. 119:804–811.
Seligmann, B. E., E. K. Gallin, D. L. Martin, W. Shain, andJ. I. Gallin. 1980. Interaction of chemotactic factors with human polymorphonuclear leukocytes: Studies using a membrane potential-sensitive cyanine dye.J Membr. Biol. 52:257–272.
Zigmond, S. H., A. Woodworth, andG. Daukas. 1985. Effects of sodium on chemotactic peptide binding to polymorphonuclear leukocytes.J. Immunol. 135:531–536.
Kuroki, M., N. Kamo, Y. Kobatake, E. Okimasu, andK. Utsumi. 1982. Measurement of membrane potential in polymorphonuclear leukocytes and its changes during surface stimulation.Biochim. Biophys. Acta 693:326–334.
Roberts, R. L., N. L. Mounessa, andJ. I. Gallin. 1984. Increasing extracellular potassium causes calcium-dependent shape change and facilitates concanavalin A capping in human neutrophils.J. Immunol. 132:2000–2006.
Zimmerli, W., B. Seligmann, andJ. I. Gallin. 1986. Exudation primes human and guinea pig neutrophils for subsequent responsiveness to the chemotactic peptide fmet-leu-phe and increases C3bi receptor expression.J. Clin. Invest. 77:925–33.
Zimmerli, W., F. A. Waldvogel, P. Vaudaux, andU. E. Nydegger. 1982. Pathogenesis of foreign body infection: Description and characteristics of an animal model.J. Infect. Dis. 146:487–97.
Lowry, O. H., N. J. Rosenbrough, A. L. Farr, andR. J. Randall. 1951. Protein measurement with the folin phenol reagent.J. Biol. Chem. 193:265–75.
Pruett, T. L., D. E. Chenoweth, V. D. Fiegel, R. D. Nelson, andR. L. Simmons. 1985.Escherichia coli and human neutrophils.Arch. Surg. 120:212–16.
Gallin, E. 1986. Ionic channels in leukocytes.J. Leuk. Biol. 39:241–54.
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Zimmerli, W., Gallin, J.I. Pus potassium. Inflammation 12, 37–43 (1988). https://doi.org/10.1007/BF00915890
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DOI: https://doi.org/10.1007/BF00915890