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Modelluntersuchungen zur papainkatalysierten Peptidsynthese im wäßrig-organischen Zweiphasensystem

Model studies on papain-catalyzed peptide synthesis in a biphasic aqueous-organic system

  • Organische Chemie und biochemie
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Abstract

Several model peptides have been synthesized enzymatically using papain as a catalyst in biphasic aqueous-organic systems. The effect of different cosolvents,pH, buffer concentration, and reaction time on the papaincatalyzed synthesis was examined. A comparison of the results obtained indicates that water-immiscible organic solvents provide higher yields than methanol in papain mediated peptide synthesis with carboxyl components in the carboxyl free form. Furthermore, it could be established that papaincatalyzed peptide synthesis can be considerably speeded up by employing acyl peptide esters instead of acyl peptides. The former should promote the rapid formation of the acyl-enzyme intermediate.

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Authors and Affiliations

  1. Bereich Biochemie, Sektion Biowissenschaften der Karl-Marx-Universität, DDR-7010, Leipzig, Deutsche Demokratische Republik

    Günter Döring, Peter Kuhl & Hans-Dieter Jakubke

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  1. Günter Döring
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  2. Peter Kuhl
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  3. Hans-Dieter Jakubke
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Döring, G., Kuhl, P. & Jakubke, HD. Modelluntersuchungen zur papainkatalysierten Peptidsynthese im wäßrig-organischen Zweiphasensystem. Monatshefte für Chemie 112, 1165–1173 (1981). https://doi.org/10.1007/BF00905471

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  • DOI: https://doi.org/10.1007/BF00905471

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