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Effect of intracellular 2,4-D concentration on plantlet regeneration of rice (Oryza sauva L.) callus

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Abstract

Intracellular 2,4-dichlorophenoxyacetic acid (2,4-D) concentration profiles in rice (Oryza sativa L.) callus were measured for various subculture periods. 2,4-D was taken up into the callus by uphill transportation from the medium. The intracellular 2,4-D taken up was metabolized gradually during cultivation. The intracellular 2,4-D concentration markedly affected the redifferentiation process after subculture in such a way that the higher its concentration was, the more strongly the redifferentiation process was suppressed, and the lesser was the browning of the callus.

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Author notes

  1. K. Mitsuoka

    Present address: New Material Division, Kanebo Limited, R&D Laboratory, 306-02, Ibaraki, Japan

  2. H. Honnda

    Present address: Department of Biotechnology, Faculty of Engineering, Nagoya University, 464-01, Nagoya, Japan

Authors and Affiliations

  1. Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, 227, Yokohama, Japan

    K. Mitsuoka, H. Honnda, X. -H. Xing & H. Unno

Authors
  1. K. Mitsuoka
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  2. H. Honnda
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  3. X. -H. Xing
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  4. H. Unno
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Mitsuoka, K., Honnda, H., Xing, X.H. et al. Effect of intracellular 2,4-D concentration on plantlet regeneration of rice (Oryza sauva L.) callus. Appl Microbiol Biotechnol 42, 364–366 (1994). https://doi.org/10.1007/BF00902743

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  • DOI: https://doi.org/10.1007/BF00902743

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