Abstract
The effects were examined of 6-month intermittent hypobaric (4000 m) exposure on the antioxidant enzyme systems in soleus and tibialis muscles of rats. At the end of the 6-month experimental exposure, the six rats in both the exposed group and the control group were sacrificed. Immunoreactive mitochondrial superoxide dismutase (Mn-SOD) contents were measured as well as the activities of antioxidant enzymes [Mn-SOD, cytosolic SOD (Cu,Zn-SOD), catalase (CAT), and glutathione peroxidase (GPX)]. Thiobarbituric acid-reactive substances (TBARS) were also determined as an indicator of lipid peroxidation. The high altitude exposure resulted in a marked increase in TBARS content in soleus muscle, suggesting increased levels of oxygen free radicals. Conversely, significant decreases in both Mn-SOD content and activity in solens muscle were oted affer exposure. Such trends were not noticed in tibialis muscle. On the other hand, no significant changes in Cu,Zn-SOD, CAT, or GPX were observed in either muscle. These results suggested that the increases in lipid peroxidation were most probably a result of decreased Mn-SOD function which was more depressed in oxidative than in glycolytic muscle.
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References
Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287
Costa LE (1990) Hepatic cytochrome P-450 in rats submitted to chronic hypobaric hypoxia. Am J Physiol 259: C654–C659
De Groot H, Littauer A (1989) Hypoxia, reactive oxygen and cell injury. Free Radic Biol Med 6:541–551
Dhalival H, Kirshenbaum LA, Randhawa AK, Signal PK (1991) Correlation between antioxidant changes during hypoxia and reoxygenation. Am J Physiol 261: H632–H638
Gregory EM, Fridovich I (1973) Oxygen toxicity and the superoxide dismutase. J Bacteriol 114:1197–1201
Hoshida S, Kuzuya T, Fuji H, Yamashita N, Oe H, Hori M, Suzuki K, Taniguchi N, Tada M (1993) Sublethal ischemia alters myocardial antioxidant activity in canine heart. Am J Physiol 264: H33–H39
Laughlin MH, Armstrong RB (1982) Muscular blood flow distribution patterns as a function of running speed in rats. Am J Physiol 243: H296–H306
Liu J, Simon LM, Phillips JR, Robin ED (1977) Superoxide dismutase (SOD) activity in hypoxic mamalian system. J Appl Physiol 42:107–110
Matsuda Y, Higashiyama S, Kijima Y, Suzuki K, Akiyama M, Kawano K, Kawata S, Tarui S, Deutsch HF, Taniguchi N (1990) Preparation, subunit structure and sulfhydryl reactivity of human manganase superoxide dismutase. Eur J Biochem 194:713–720
McCutchan HJ, Schwappach JR, Enquist EG, Walden DL, Terade LS, Reiss OK, Leef JA, Repine JE (1990) Xanthine oxidase-derived H2O2 contributes to reperfusion injury of ischemic skeletal muscle. Am J Physiol 258: H1415–H1419
Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358
Ohno H, Yahata T, Yamashita K, Yamamura K, Sato Y, Yamamoto C, Hayamizu S, Taniguchi N (1988) Effects of physical training on immunoreactive γ-glutamyltransferase in human plasma. Enzyme 39:110–114
Ohno H, Yamashita H, Ookawara T, Saito D, Wakabayashi K, Taniguchi N (1992) Training effects on concentrations of immunoreactive superoxide dismutase isoenzyme in human plasma. Acta Physiol Scand 146:291–292
Rice CL, Pettgrew FP, Noble EG, Taylor AW (1988) The fibre composition of skeletal muscle. In: Poortmans JR (ed) Principles of exercise biochemistry. Karger, Basel, pp 22–23
Sen CK, Ookawara T, Suzuki K, Taniguchi N, Hänninen O, Ohno H (1994) Immunoreactivity and activity of mitochondrial superoxide dismutase following training and exercise. Pathophysiology 00:1–4
Srivastava G, Bhatnagar R, Viswanathan R, Venkintasubramanian TA (1980) Microsomal and mitochondrial cytochromes in acutely hypoxic rat lung and liver. Ind J Biochem Biophys 17:130–134
Suzuki K, Nakata T, Seo HG, Miyazawa N, Sugiyama T, Taniguchi N (1991) A new model for hepatitis and liver cancer. In: Mori M, Yoshida MC, Takeichi N, Taniguchi N (eds) The EC rats. Springer, Berlin, Heidelberg, New York, Tokyo, pp 142–148
Suzuki K, Kinoshita N, Matsuda Y, Higashiyama S, Kuzuya T, Minamino T, Tada M, Taniguchi N (1992) Elevation of immunoreactive serum Mn-Superoxide dismutase in patients with acute myocardial infarction. Free Radic Res Commun 6:325–334
Taniguchi N (1992) Clinical significances of superoxide dismutases: changes in aging, diabetes, ischemia, and cancer. Adv Clin Chem 29:1–47
Tappel AL (1978) Glutathione peroxidase and hydroperoxidase. In: Fleischer S, Packer L (eds) Methods Enzymol 52:506–513
Turrens JF, Alexandre A, Lehninger AL (1985) Ubisemiquinone is an electron donor for superoxide formation by complex III of heart mitochondria. Arch Biochem Biophys 237:408–414
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Radák, Z., Lee, K., Choi, W. et al. Oxidative stress induced by intermittent exposure at a simulated altitude of 4000 m decreases mitochondrial superoxide dismutase content in soleus muscle of rats. Europ. J. Appl. Physiol. 69, 392–395 (1994). https://doi.org/10.1007/BF00865401
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DOI: https://doi.org/10.1007/BF00865401