Abstract
The solubilization of Fe(III) hydroxide by the naturally occurring siderophoreN,N′-bis(2,3-dihydroxybenzoyl)-l-lysine has been investigated spectrophotometrically in the presence and the absence of a stoichiometric amount of molybdate in aqueous medium at pH 7. In the absence of molybdate the reaction is 50% complete after 115 min. In contrast, the addition of an equimolar amount of molybdate results in an instantaneous formation of the molybdenum siderophore complex and a significant delay in the formation of the corresponding iron complex: 50% of the iron complex is present after 44 h and equilibrium is only reached after 2 weeks. The results are discussed with regard to metal acquisition by the nitrogen fixing cells ofAzotobacter vinelandii.
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Duhme, AK., Hider, R.C. & Khodr, H. Spectrophotometric competition study between molybdate and Fe(III) hydroxide onN,N′-bis(2,3-dihydroxybenzoyl)-l-lysine, a naturally occurring siderophore synthesized byAzotobacter vinelandii . Biometals 9, 245–248 (1996). https://doi.org/10.1007/BF00817923
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DOI: https://doi.org/10.1007/BF00817923