Summary
Three related mammalian carrier proteins that mediate the transport of cationic amino acids through the plasma membrane have been identified in murine and human cells (CAT for cationic amino acid transporter). Models of the CAT proteins in the membrane suggest they have 12 or 14 transmembrane domains connected by short hydrophilic loops and intracellular N- and C-termini. The transport activity of the CAT proteins is sensitive to trans-stimulation and independent of the presence of sodium ions. These features agree with the behaviour of carrier proteins mediating facilitated diffusion. The three CAT proteins, CAT-1, CAT-2A and CAT-2(B) are encoded by two different genes (CAT-1 and CAT-2). CAT-1 and CAT-2(B) exhibit transport properties consistent with system y+, the principal mechanism for cellular uptake of cationic amino acids. In contrast, CAT-2A has tenfold lower substrate affinity, greater apparent maximal velocity and it is much less sensitive to trans-stimulation. In addition to structural and functional aspects, this review discusses the role of the CAT proteins for supplying substrate to NO synthases and the property of the rodent CAT-1 proteins to function as virus receptors.
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Abbreviations
- CAT:
-
cationic amino acid transporter
- m:
-
mouse
- h:
-
human
- r:
-
rat
- Tea:
-
T cell early activation protein
- CAA:
-
cationic amino acids
- TM:
-
transmembrane spanning domain
- rBAT:
-
related to b0,+ amino acid transporter
- 4F2hc:
-
4F2 heavy chain cell surface antigen
- MuLV:
-
murine leukemia viruses
- Km :
-
Michaelis Menten constant
References
Albritton LM, Tseng L, Scadden D, Cunningham JM (1989) A putative murine ectropic retrovirus receptor gene encodes a multiple membrane-spanning protein and confers susceptibility to virus infection. Cell 57: 659–666
Albritton LM, Bowcock AM, Eddy RL, Morton CC, Tseng L, Farrer LF, Cavalli-Sforza LL, Shows TB, Cunningham JM (1992) The human cationic amino acid transporter (ATRC1): physical and genetic mapping to 13q12–q14. Genomics 12: 430–434
Albritton LM, Kim JW, Tseng L, Cunningham J (1993) Envelope binding domain in the cationic amino acid transporter determines the host range of ecotropic murine retroviruses. J Virol 67: 2091–2096
Bertran J, Testar X, Zorzano A, Palacin M (1994) A new age for mammalian plasma membrane amino acid transporters. Cell Physiol Biochem 4: 217–241
Bogle RG, Baydoun AR, Moncada S, Pearson JD, Mann GE (1992) L-arginine transport is increased in macrophages generating nitric oxide. Biochem J 284: 15–18
Bull HB, Breese K (1974) Surface tension of amino acid solutions: a hydrophobicity scale of the amino acid residues. Arch Biochem Biophys 161: 665–670
Christensen HN (1964) A transport system serving for mono- and diamino acids. Proc Natl Acad Sci USA 51: 337–344
Christensen HN (1990) Role of amino acid transport and countertransport in nutrition and metabolism. Physiol Rev 70: 43–77
Christensen HN, Antonioli JA (1969) Cationic amino acid transport in the rabbit reticulocyte. J Biol Chem 244: 1497–1504
Closs EI, Albritton LM, Kim JW, Cunningham JM (1993a) Identification of a low affininty, high capacity transporter of cationic amino acids in mouse liver. J Biol Chem 268: 7538–7544
Closs EI, Borel Rinkes I, Bader A, Yarmush M, Cunningham J (1993b) Retroviral infection and the expression of cationic amino acid transporters in rodent hepatocytes. J Virol 67: 2097–2102
Closs EI, Lyons CR, Kelly C, Cunningham JM (1993c) Characterization of the third member of the MCAT family of cationic amino acid transporters — identification of a domain that determines the transport properties of the MCAT proteins. J Biol Chem 268: 20796–20800
Deves R, Angelo S, Chavez P (1993) N-Ethylmaleimide discriminates between two lysine transport systems in human erythrocytes. J Physiol-London 468: 753–766
Eisenberg D, Schwarz E, Komaromy M, Wall R (1984) Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J Mol Biol 179: 125–142
Harvey CM, Muzyka WR, Yao SYM, Cheeseman CI, Young JD (1993) Expression of rat intestinal L-lysine transport systems in isolated oocytes of Xenopus laevis. Am J Physiol 265: G99-G106
Hibbs JJ, Vavrin Z, Taintor RR (1987) L-arginine is required for expression of the activated macrophage effector mechanism causing selective metabolic inhibition in target cells. J Immunol 138: 550–565
Hopp TP, Woods KR (1981) Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci USA 78: 3824–3828
Kakuda DK, Finley KD, Dionne VE, MacLeod CL (1993) Two distinct gene products mediate y+ type cationic amino acid transport in Xenopus oocytes and show different tissue expression patterns. Transgene 1: 91–101
Kavanaugh MP (1993) Voltage dependence of facilitated arginine flux mediated by the system y+ basic amino acid transporter. Biochemistry 32: 5781–5785
Kavanaugh MP, Miller DG, Zhang WB, Law W, Kozak SL, Kabat D, Miller AD (1994a) Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters. Proc Natl Acad Sci USA 91: 7071–7075
Kavanaugh MP, Wang H, Zhang Z, Zhang W, Wu YN (1994b) Control of cationic amino acid transport and retroviral receptor functions in a membrane protein family. J Biol Chem 269: 15445–15450
Kilberg MS, Stevens BR, Novak DA (1993) Recent advances in mammalian amino acid transport. Annu Rev Nutr 13: 137–165
Kim JW, Cunningham JM (1993) N-Linked glycosylation of the receptor for murine ecotropic retroviruses is altered in virus-infected cells. J Biol Chem 268: 16316–16320
Kim JW, Closs EI, Albritton LM, Cunningham JM (1991) Transport of cationic amino acids by the mouse ecotropic retrovirus receptor. Nature 352: 725–728
Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157: 105–132
MacLeod CL, Finley K, Kakuda D, Kozak CA, Wilkinson MF (1990) Activated T cells express a novel gene on chromosome 8 that is closely related to the murine ectropic retroviral receptor. Mol Cell Biol 10: 3663–3674
Manavalan P, Ponnuswamy PK (1978) Hydrophobic character of amino acid residues in globular proteins. Nature 275: 673–674
Miller DG, Edwards RH, Miller AD (1994) Cloning of the cellular receptor for amphotropic murine retroviruses reveals homology to that for gibbon ape leukemia virus. Proc Natl Acad Sci USA 91: 78–82
O'Hara B, Johann SV, Klinger HP, Blair DG, Rubinson H, Dunn KJ, Sass P, Vitek SM, Robbins T (1990) A gene conferring sensitivity to infection by GALV. Cell Growth Differ 1: 119–127
Palacin M (1994) A new family of proteins (rBAT and 4F2hc) involved in cationic and zwitterionic amino acid transport: a tale of two proteins in search of a transport function. J Exp Biol 196: 123–137
Palmer RM, Ashton DS, Moncada S (1988) Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature 333: 664–666
Puppi M, Henning SJ (1995) Cloning of the rat ecotropic retroviral receptor and studies of its expression in intestinal tissues. Proc Soc Exp Biol Med 209: 38–45
Reizer J, Finley K, Kakuda D, MacLeod C, Reizer A, Saier M (1993) Mammalian integral membrane receptors are homologous to facilitators and antiporters of yeast, fungi and eubacteria. Prot Science 2: 20–30
Somia NV, Zoppe M, Verma IM (1995) Generation of targeted retroviral vectors by using single-chain variable fragment: an approach to in vivo gene delivery. Proc Natl Acad Sci USA 92: 7570–7574
Sophianopoulou V, Diallinas G (1995) Amino acid transporters of lower eukaryotes: regulation, structure and topogenesis. FEMS Microbiol Rev 16: 53–75
Van Winkle LJ (1993) Endogenous amino acid transport systems and expression of mammalian amino acid transport proteins in Xenopus oocytes. Biochem Biophys Acta 1154: 157–172
Van Winkle LJ, Campione AL (1990) Functional changes in cation-preferring amino acid transport during development of preimplantation mouse conceptuses. Biochim Biophys Acta 1028: 165–173
Van Winkle LJ, Christensen HN, Campione AL (1985) Na+-dependent transport of basic, zwitterionic, and bicyclic amino acids by a broad scope system in mouse blastocytes. J Biol Chem 260: 12118–12123
Van Winkle LJ, Campione AL, Gorman JM (1988) Na+-independent transport of basic and zwitterionic amino acids in mouse blastocytes by a shared system and by processes which distinguish between these substrates. J Biol Chem 263: 3150–3163
Vanzeijl M, Johann SV, Closs E, Cunningham J, Eddy R, Shows TB, O'Hara B (1994) A human amphotropic retrovirus receptor is a 2nd member of the gibbon ape leukemia virus receptor family. Proc Natl Acad Sci USA 91: 1168–1172
Wang H, Kavanaugh MP, North RA, Kabat D (1991) Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter. Nature 352: 729–731
Wang H, Dechant E, Kavanaugh M, North RA, Kabat D (1992) Effects of ecotropic murine retroviruses on the dual-function cell surface receptor/basic amino acid transporter. J Biol Chem 267: 23617–23624
Wang H, Kavanaugh P, Kabat D (1994) A critical site in the cell surface receptor for ecotropic murine retroviruses required for amino acid transport but not for viral reception. Virology 202: 1058–1060
Weiss RA (1993) Cellular receptors and viral glycoproteins involved in retroviral entry. In: The retroviridae. Plenum Press, New York, pp 1–108
White MF (1985) The transport of cationic amino acids across the plasma membrane of mammalian cells. Biochim Biophys Acta 822: 355–374
White MF, Christensen HN (1982) Cationic amino acid transport into cultured animal cells: II. Transport system barely perceptible in ordinary hepatocytes, but active in hepatoma cell lines. J Biol Chem 257: 4450–4457
Woodard MH, Dunn WA, Laine RO, Malandro M, McMahon R, Simell O, Block ER, Kilberg MS (1994) Plasma membrane clustering of system y(+) (CAT-1) amino acid transporter as detected by immunohistochemistry. Am J Physiol 266: E817-E824
Wu JY, Robinson D, Kung HJ, Hatzoglou M (1994) Hormonal regulation of the gene for the type C ecotropic retrovirus receptor in rat liver cells. J Virol 68: 1615–1623
Yoshimoto T, Yoshimoto E, Meruelo D (1991) Molecular cloning and characterization of a novel human gene homologous to the murine ecotropic retroviral receptor. Virology 185: 10–15
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Closs, E.I. CATs, a family of three distinct mammalian cationic amino acid transporters. Amino Acids 11, 193–208 (1996). https://doi.org/10.1007/BF00813860
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DOI: https://doi.org/10.1007/BF00813860