Abstract
From the present review integrating old and new data emerge a few principles of gene expression in eukaryotes, and an infinite variety of possible mechanistic details generating the overal pattern. The few principles, most of which are not fundamentally new, may thus be summarized. 1) The eukaryotic genome is subdivided into transcriptional units: intoTranscriptons which are subject to individual activation controlled at DNA level. 2) Viral genomes may contain one or a few transcriptons, while cells of multicellular organisms contain from 3×103 in diptera up to an estimated 2×105 in birds and mammals. 3) Transcriptons may include one or several coding sequences. 4) Transcriptons vary considerably in size: in mammals and birds their size spectrum falls into the 2,000 to 20,000 bp range. 5) Units of coding information constituting one message (genes) and, possibly, units of regulative information are frequently broken up and stored within the transcripton in sub-genic blocks (of so far unknown significance) in general located at a certain distance from the 5′ and 3′ transcript terminals which are determined by the promotor and terminator signals. 6) The gene, in its specific definition as the functional unit underlying the phenotype, is in general constituted postranscriptionally by the processing mechanisms from the mosaic of its genomic subunits in the transcripton; segments of coding, service and regulative sequences are recombined within themselves and with each other, polygenic transcripts separate into their unit messages. 7) Activated transcriptons produce pre-mRNA; these primary transcripts are colinear with the DNA of the transcriptional unit. 8) Primary pre-mRNA is processed into secondary pre-mRNA's by extragenic cleavage and intragenic (“splicing”) processing, giving rise stepwise to functional mRNA. During this process chemical modifications as methylation, 5′-terminal capping and 3′-terminal polyadenylation take place. 9) Translation yields either potentially functional polypeptides or polycistronic polyproteins subject to further processing. 10) Processing is a regulated process; it involves many of the possible phases and mechanisms of post-transcriptional regulation (cf. 39, 40).
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Klessig, D.F. Cell 12: 9 (1977).
Scherrer, K., Marcaud, L., Zajdela, F., London, I. and Gros, F. Proc. Nat. Acad. Sci. USA 56: 1571 (1966). Scherrer, K., Marcaud, L., Zajdela, F., Breckenridge, B. and Gros, F. Bull. Soc. Chim. Biol. 48: 1037 (1966).
Darnell, J.E. In: The Harvey Lecture Series 69. Acad. Press, New-York, p. 1 (1974).
Darnell, J.E. Prog. Nucl. Acid Res. and Mol. Biol. 22: 327 (1978).
Lewin, B. Cell 4: 11 (1975).
Perry, R.P. Ann. Rev. Biochem. 45: 605 (1976).
Perry, R.P. In: E.R. Dirksen, D. Prescott and C.F. Fox (Eds). Cell Reproduction: Daniel Mazia Dedicatory Volume (ICN-UCLA conf.) In Press.
Molloy, G., and Puckett, L., Prog. Biophys. Mol. Biol. 31: 1 (1976).
Jacob, F. and Monod, J. J. Mol. Biol. 3: 318 (1961).
Sibatini, A., De Kloet, S.R., Allfrey, V.G. and Mirsky, A.E. Biochemistry 48: 471 (1962).
Georgiev, G.P., Samarina, O.P., Lerman, M.L., Smirnov, M.N. and Severtzov, A.N. Nature 200: 1291 (1963).
Scherrer, K., Latham, H. and Darnell, J.E. Proc. Nat. Acad. Sci. USA 49: 240 (1963).
Gros, F. Hiatt, H., Gilbert, W., Kurland, C.G. Rosebrough, R.W. and Watson, J.D. Nature 90: 581 (1961).
Perry, R.P. Proc. Nat. Acad. Sci. USA 48: 2179 (1962).
Scherrer, K. and Darnell, J.E. Biochem. Biophys. Res. Comm. 7: 486 (1962).
Weinberg, R., Loening, U., Willems, M. and Penman, S. Proc. Nat. Acad. Sci. USA 58: 1088 (1967).
Nikolaev, N., Birzge, C.H., Gotoh, S., Glazier, K., Schlessinger, D. Brookhaven Symposium in Biology. Processing of RNA, 26: 175 (1975).
Granboulan, N. and Scherrer, K. Eur. J. Biochem. 9: 1 (1969).
Soeiro, R. Birnboim, H.C. and Darnell, J.E. J. Mol. Biol. 19: 362 (1966).
Attardi, G., Parnas, H., Hwang, M.I.H. and Attardi, B. J. Mol. Biol. 20: 145 (1966).
Cox, R.A. Biochem. J. 114: 753 (1969).
Scherrer, K. In: Habel and Salzman (Eds). Fundamental Technique in Virology. Acad. Press, p. 413 (1969).
MacNaughton, M., Freeman, K.B. and Bishop, J.O. Cell 1: 117 (1974).
Brawerman, G., Biezunski, N. and eisenstadt, J. Biochim. Biophys. Acta 103: 201 (1965).
Fedoroff, N., Wellauer, P.K., Wall, R. Cell 10: 597 (1977).
Jelinek, W. and Darnell, J.E. Proc. Nat. Acad. Sci. USA 69: 2637 (1972).
ryskov, A.P., Saunders, G.F., Farashyan, V.R. and Georgiev, G.P. Biochim. Biophys. Acta 312: 152 (1973).
Scherrer, K. and Marcaud, L. J. Cell. Physiol. 72: 181 (1968).
Edström, J.E. and Daneholt, B. J. Mol. Biol. 28: 331 (1967).
Scherrer, K., spohr, G., Granboulan, N., Morel, C., Grosclaude, J. and Chezzi, C. Cold Spring Harbour Symp. Quant Biol. 35, 539 (1970).
Wellauer, P.K. and Dawid, I.B. Cell 10: 193 (1977).
Celma, M.L., Dhar, R., Pan, J. and Weissman, S.M. Nuc. Acids Res. 4: 2549 (1977).
Aloni, Y., Dhar, R., Laub, O., Horowitz, M. and Khoury, G. Proc. Nat. Acad. Sci. USA 74: 3686 (1977).
Berget, s.M., Moore, C. and Sharp, P.A. Proc. Nat. Acad. Sci. USA 74: 3171 (1977).
Chow, L.T., Gelinas, R.E., Broker, T.R. and Roberts, R.J. Cell 12: 1 (1977).
Breathnack, R., Mandel, J.L. and Chambon, P. Nature 270: 314 (1977).
Jeffreys, A.J. and flavell, R.A. Cell 12: 1097 (1977).
Tilghman, S.M., Tiemeier, D.C., Seidman, J.G., Peterlin, B.M., Sullivan, M., Maizel, J.V. and Leder, P. Proc. Nat. Acad. Sci. USA 75: 725 (1978).
Brack, C. and tonegawa, S. Proc. Nat. Acad. Sci. USA 74: 5652 (1977).
Scherrer, K., In: E. Diczfalusy (ed.) Protein Synthesis in Reproductive Tissue. 6th Karolinska Symposium on Research Methods in Reproductive Endocrinology. Bogtrykkeriet Forum, Copenhagen, p. 95 (1973).
Scherrer, K. In: Kohn and Schatkay (Eds). Strategies for the Control of Gene Expression. 18th Oholo Annual Biological Conference. Plenum Press, New-York, p. 169 (1974).
Scherrer, K. In: Kolodny (ed). Eukaryotic Gene Regulation. CRC Press, in press (1979).
Szibalsky, W., Bure, K., fiandt, M., Hayes, S., Hradecna, Z., Kumar, S., Lozeron, H.A., Nijkamp, H.J.J., and Stevens, W.F. Cold Spring Harbour Symp. Quant. Biol. 35: 341 (1970).
Ruiz-Carillo, A., Beato, M., Schutz, G., Feigelson, D. and Allfrey, G. Proc. Nat. Acad. Sci. USA 70: 3641 (1973).
Knöchel, W., and tiedemann, H. Biochim. Biophys. Acta 378: 383 (1975).
Fox, C.F., Robinson, W.S., Haselkorn, R. and Weiss, S.B. J. Biol. Chem. 239: 186 (1964).
Pemberton, R.E. and Melli, M. Nature New Biol. 236: 172 (1972).
Strauss, J.H. Jr., Kelly, R.B., and Sinsheimer, R.L. Biopolymers 6: 793 (1968).
Spohr, G., Mirault, M-E., Imaizumi, M-T. and Scherrer, K. Eur. J. Biochem. 62: 313 (1976).
Bailey, J.M., and Davidson, N. Anal. Biochem. 70: 75 (1976).
Mirault, M-E. and Scherrer, K., Eur. J. Biochem. 23: 372 (1971).
Ladhoff, A-M., Thiele, B.J. and Coutelle, C. Eur. J. Biochem. 58: 431 (1975).
Spohr, G., Imaizumi, M-T. and Scherrer, K. Proc. Nat. Acad. Sci. USA 71: 5009 (1974).
Spohr, G., Dettori, G., Manzari, V., Cell 8: 505 (1976).
Baltimore, D. Nature 226: 1209 (1970).
Kelly, R.B., Cozzarelli, N.R., Deutscher, M.P., Lehman, I.R. and Kornberg, A. J. Biol. Chem. 246: 39 (1970).
Paterson, B.M., Roberts, B.E. and Kuff, E.L. Proc. Nat. Acad. Sci. USA 74: 4370 (1977).
Haines, J.R., Kalb, V.F., Rosteck, P. and Lingrel, J.B. FEBS Lett. 91: 173 (1978).
Imaizumi, T., Diggelman, H. and Scherrer, K. Proc. Nat. Acad. Sci. USA 70: 1122 (1973).
Ross, J. J. Mol. Biol. 106: 403 (1976).
Puvion-Duthilleul, F., Bernadac, A. Puvion, E. and Bernhard, W. J. Ultrastr. Res. 58: 108 (1977).
Bishop, J.O., Cell 2: 81 (1974).
Zan-Kowalczewska, M., Bretner, M., Sierakowska, H., Szczesna, E., Filipowicz, W. and Shatkin, A.J. Nucleic Acids Res. 4: 3065 (1977).
Moss, B., Martin, S.A., Ensinger, M.J., Boone, R.F. and Wei, C.H. Progr. Nucl. Acid. Res. Mol. Biol. 19: 63 (1976).
Spencer, E., Loring, D., Hurwitz, J., and Monroy, G. Proc. Natl. Acad. Sci. USA 75: 4793 (1978).
Schibler, U. and Perry, R.P. Cell 9: 121 (1976).
Villareal, L.P., While, R.T. and Berg, P. Virology 24: 209 (1979).
Reynaud, C.A., Imaizumi Scherrer, T., and Scherrer, K. Submitted to J. Mol. Biol.
Bastos, r.N. and Aviv, H. Cell 11: 641 (1977).
Curtis, P.J. and Weissmann, C. J. Mol. Biol. 106: 1061 (1976).
Curtis, P.J., Mantei, N., van den Berg, J. and Weissmann, C. Proc. Nat. Acad. Sci. USA 74: 3184 (1977).
Kwan, S-P., Wood, T.G., and Lingrel, J.B. Proc. Nat. Acad. Sci. USA 74: 178 (1977).
Lehrach, H., Diamond, D., Wozney, J.M. and Boedtker, H. Biochemistry 16: 4743 (1977).
Strair, R.K., skoultchi, A.I. and Shafritz, D.A. Cell 12: 133 (1977).
Knöchel, W. and Grundmann, U. Biochim. and Biophys. Acta 517: 99 (1978).
Tilghman, S.M., Curtis, P.J., Tiemeier, D.C., Leder, P. and Weissmann, C. Proc. Nat. Acad. Sci. USA 75: 1309 (1978).
Williamson, R., Drewienkiewicz, C.E. and Paul, J. Nature New Biol. 241: 66 (1973).
Therwath, A. and Scherrer, K. Proc. Nat. Acad. Sci. USA 75: 3776 (1978).
Mandel, J.L., Breathnach, R. Gerlinger, P., Le Meur, M., Gannon, F. and Chambon, P. Cell 14: 641 (1978).
Old, R.W., Callan, H.G. and Gross, K.W. J. Cell Sci. 27: 57 (1977).
Flavell, R.A., Kooter, J.M., de Boer, E., Little, P.F.R. and Williamson, R. Cell 15: 25 (1978).
Levy, S., Childs, G. and Kedes, L. Cell 15: 151 (1978).
Hackett, P.B., Traub, P., and Gallwitz, D. J. Mol. Biol. 125: 619 (1978).
Melli, M. Spinelli, G., Wyssling, H. and Arnold, E. Cell 11: 651 (1977).
Kunkel, N.S., Hemminki, K. and Weinberg, E.S. Biochemistry 17: 2591 (1978).
Cohen, L.H., Newrock, K.M. and Zweidler, A. Science 190: 994 (1975).
Lizardi, P.M. and Brown, D.D. Cell 4: 207 (1975).
Case, S.T. and Daneholt, B. J. Mol. Biol. 124: 223 (1978).
Serfling, E. and Huth, A. Chromosoma 66: 205 (1978).
Egyhazi, E. Chromosoma 65: 137 (1978).
Suzuki, Y. and Ohshima, Y. Cold Spring Harbour Symp. Quant. Biol. 42: 947 (1977).
Tsujimoto, Y., and Suzuki, Y., Cell 16: 425 (1979).
Brack, C., Hirama, M., Lenhard-Schueller, R. and Tonegawa, S. Cell 15: 1 (1978).
Gilmore-Hebert, M. and Wall, R. Proc. Nat. Acad. Sci. USA 75: 342 (1978).
Schibler, U., Marcu, K.B. and Perry, R.P. Cell 15: 1495 (1978).
Engel, J.D. and Dodgson, J.B. J. Biol. Chem. 253: 8239 (1978).
Strair, R.K., Yap, S.H., Nadal-Girard, B. and Shafritz, D.A. J. Biol. Chem. 253: 1328 (1978).
Weinstock, R., Sweet, R., Weiss, M., Cedar, H. and Axel, R. Proc. Nat. Acad. Sci. USA 75: 1299 (1978).
Doel, M.T., Houghton, M., Cook, E.A. and Carey, N.H. Nuc. Acids Res. 4: 3701 (1977).
MacKnight, G.S., and Schimke, R.T. Proc. Nat. Acad. Sci. USA 71: 4327 (1974).
Roop, D.R., Nordstrom, J.L., Tsai, S.Y., Tsai, M-J., and O'Malley, B.W. Cell 15: 671 (1978).
Sauerbier, W., Millette, R.L. and Hackett, P.B. Jr. Biochim. Biophys. Acta 209: 368 (1970).
Giorno, R. and Sauerbier, W. Cell 9: 775 (1976).
Goldberg, S., Schwartz, H. and Darnell, J.E. Proc. Nat. Acad. Sci USA 74: 4520 (1977).
Herman, R.C., Williams, J.G. and Penman, S. Cell 7: 429 (1976).
Hames, B.D. and Perry, R.P. J. Mol. Biol. 109: 437 (1977).
Shearer, R.W. and McCarthy, B.J. Biochemistry 6: 283 (1967).
Shearer, R.W. and McCarthy, B.J. J. Cell Physiology 75: 97 (1970).
Hough, B.R., Smith, M.J., Britten, R.J. and Davidson, E.H. Cell 5: 291 (1975).
Harpold, M., Evans, R., Salditt-Georgieff, M., and Darnell, J.E., in preparation.
Acheson, N.H., buetti, E., Scherrer, K. and Weil, R. Proc. Nat. Acad. Sci. USA 68: 2231 (1971).
Lindberg, U. and Darnell, J.E. Proc. Nat. Acad. Sci. USA 65: 1089 (1976).
Acheson, N.H. Proc. Nat. Acad. Sci. USA 75: 4754 (1978).
Aloni, Y. Proc. Nat. Acad. Sci. USA 69: 2404 (1972).
Lindstrom, D.M. and Dulbecco, R. Proc. Nat. Acad. Sci. USA 69: 1517 (1972).
Khoury, G., Burne, J.C. and Martin, M.A. Proc. Nat. Acad. Sci. USA 69: 1925 (1972).
Green, M., Parson, J.T., Pina, M. Fujinaga, K., Caffier, H. and Landgraf-Lewis, I. Cold Spring Harbour Symp. Quant. Biol. 35: 803 (1970).
Philipson, L., Pettersson, U., Lindberg, U., Tibbets, C., Vennström, B. and Persson, T. Cold Spring Harbour Symp. Quant. Biol. 39: 447 (1974).
Mulder, C., Arrand, J.R., Delius, H., Keller, W., Pettersson, U. Roberts, R.J. and Sharp, P.A. Cold Spring Harbour Symp. Quant. Biol. 39: 397 (1974).
Sharp, P.A., Gallimore, P.H. and Flint, S.J. Cold Spring Harbour Symp. Quant. Biol. 39: 457 (1974).
Bachenheimer, S. and Darnell, J.E. Proc. Nat. Acad. Sci. USA 72: 4445 (1975).
Meissner, H.C., Meyer, J., Maizel, J.V. Jr. and Westphal, H. Cell 10: 225 (1977).
Kitchingham, G.R., Lai, S-P. and Westphal, H. Proc. Nat. Acad. Sci. USA 74: 4392 (1977).
Berk, A.J. and Sharp, P.A. Cell 14: 695 (1978).
Blanchard, J.M., Weber, J., Jelinek, W. and Darnell, J.E. Proc. Nat. Acad. Sci. USA 75: 5344 (1978).
Nevins, J.R. and Darnell, J.E. J. Virol. 25: 811 (1978).
Nevins, J.R. and Darnell, J.E. Cell 15: 1477 (1978).
Ziff, E. and Fraser, N. Cell 16: 000 (1979).
Fried, M. and Griffin, B.E. Adv. Cancer Res. 24: 67 (1977).
Reddy, V.B., Thimmappaya, B., Dhar, R., Subramanian, K.N., Zain, B.S. Pan, J., ghosh, P.K., Celma, M.L. and Weissman, S.M. Science 200: 494 (1978).
Fiers, W., Contreras, R., Haegeman, G., Rogiers, R., van de Voorde, A., Heuverswyn, H., van Herreweghe, J., Volckaert, G. and Ysebaert, M. Nature 273: 113 (1978).
HSU, M-T. and Ford, J. Proc. Nat. Acad. Sci. USA 74: 4982 (1977).
Lavi, S. and Groner, Y. Proc. Nat. Acad. Sci. USA 74: 5323 (1977).
Bratosin, S., Horowitz, M., Laub, O. and Oloni, Y. Cell 13: 783 (1978).
Lai, C-J., Dhar, R., and Khoury, G. Cell 14: 971 (1978).
Gosh, P.K., Reddy, V.B., Swinscoe, J., Lebowitz, P. and Weissman, S.M. J. Mol. Biol. 126: 813 (1978).
Prives, C., gilboa, E., Revel, M., and Winocour, E. Proc. Nat. Acad. Sci. USA 74: 457 (1977).
Paucha, E., Harvey, R., Smith, R., Smith, A.E. in: May P., Monier, R. and Weil, R. (eds). “Proteins of Oncogenic DNA Viruses”, Coll. INSERM, Paris, 69: 189 (1977).
Berk, A.J. and Sharp, P.A. Proc. Nat. Acad. Sci. USA 75: 1274 (1978).
Von der Helm, K. and Duesberg, P. Proc. Nat. Acad. Sci. USA 72: 614 (1975).
Cordell, B., Weiss, S.R., Varmus, H.E. and Bishop, J.M. Cell 15: 79 (1978).
Mellon, P. and Duesberg, P.H. Nature 270: 631 (1977).
Fan, H. and Verma, I.M. J. Virol. 26: 468 (1978).
Rothenberg, E., Donoghue, D.J. and Baltimore, D. Cell 13: 435 (1978).
Baltimore, D., Jacobson, M.F., Asso, J. and Huang, A.S. Cold Spring Harbour Symp. Quant. Biol. 34: 741 (1969).
Gielkens, A.L.J., van Zaane, D., Bloemers, H.P.J. and Bloemendal, H. Proc. Nat. Acad. Sci. 73: 356 (1976).
Pawson, T., Martin, G.S. and Smith, A.E. J. Virol. 19: 950 (1976).
Jamjoom, G.A., Naso, R.B. and Arlinghaus, R.B. Virology 78: 11 (1977).
Fan, H. Curr. Top. Microbiol. Immunol. 79: 1 (1978).
Benzer, S. Ref. to be added on galley proof.
Slonimsky, P., Claisse, M.L., Foucher, M., Jacq, C., Ko Ko, A., Lamoureux, A., Pajot, R., Penodin, G., Spyridakis, A. and Wambier-Klappel, M.L. in: Bacila, M., and Horecker, B.L. (eds). “Biochemistry and Genetics of Yeast”, p. 339 (1978).
Wold, B.J., Klein, W.H. and Davidson, E.H. Cell 14: 941 (1978).
Bouloy, M., Plotch, S.J., and Krug, R.M. Proc. Natl. Acad. Sci. US 75: 4886 (1978).
Maundrell, K., Maxwell, S., Civelli, O., Vincent, A., Goldenberg, S., Buri, J-F., Imaizumi-Scherrer, M.T., and Scherrer, K. Mol. Biol. Report 5: 000 (1979).
Breathnach, R., Denvis, I.C., O'Hare, K., Gannon, F. and Chambon, P. Proc. Natl. Acad. Sci. USA 75: 4853 (1978).
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Transcripton: Having introduced at an early time the term and definition ofTranscriptional Units in order to differentiate between primary transcripts and functional mRNA (2) we think time has come to adopt the more streamline term of Transcripton which seems logical in today terms and thus fully justified. (For terminological definitions consult the Appendix to this paper, page 25).
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Scherrer, K., Imaizumi-Scherrer, MT., Reynaud, CA. et al. On pre-messenger RNA and transcriptons a review. Mol Biol Rep 5, 5–28 (1979). https://doi.org/10.1007/BF00777484
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DOI: https://doi.org/10.1007/BF00777484