Abstract
The genotoxic activity of 11 mycotoxins was investigated inEscherichia coli K 12. The induction of the SOS functionsfi A whose level of expression is monitored by means of asfi A:: lac Z operon fusion was assayed by measuring theβ-galactosidase activity in the PQ 37 strain. Most of these fungal metabolites did not induce SOS response in this bacterial test. Only aflatoxicol, a reduced metabolite of aflatoxin B1 was well detected as an SOS inducer if metabolic activation was performed. Patulin, penicillic acid and viomellein are only weak inducing agents. The other fungal compounds tested failed to demonstrate a positive SOS inducing activity. Relationship between SOS chromotest, mutagenicity toSalmonella typhimurium andin vivo carcinogenicity was discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ames BN, Durston WE, Yamasaki E, Lee FD. Carcinogens are mutagens: a simple test combining liver homogenates for activation and bacteria for detection. Proc Natl Acad Sci USA 1973; 70: 2281–2285.
Ames BN, Kammen HO, Yamasaki E. Methods for detecting carcinogens and mutagens with theSalmonella/mammalian-microsome mutagenicity test. Mutation Res 1975; 31: 347–364.
Auffray Y, Boutibonnes P. Evaluation of the genotoxic activity of some mycotoxins usingEscherichia coli in the SOS spot test. Mutation Res 1986; 171: 79–82.
Becci PJ, Voss KA, Hess FG, Gallo MA, Parent RA, Stevens KR. Long term carcinogenicity and toxicity study of zearalenone in the rat. J Appl Toxicol 1982; 2: 247–254.
Boutibonnes P, Auffray Y, Malherbe C, Kogbo W, Marais C. Propriétés antibactériennes et génotoxiques de 33 mycotoxines. Mycopathologia 1984; 87: 43–49.
Dickens F, Jones HE, Waynforth WB. Oral, subcutaneous and intratracheal administration of carcinogenic lactones and related substances: the intratracheal administration of cigarette tar in the rat. Br J Cancer 1966; 20: 134–144.
George J, Castellazzi M, Buttin G. Prophage induction and cell division inEscherichia coli. III Mutationssfi A andsfi B restore division intif andlon strains and permit expression of mutator properties oftif. Mol Gen Genet 1975; 140: 309–332.
Hayes WA. Biological activities of mycotoxins. Mycopathologia 1979; 65: 29–41.
Huisman O, D'ari R. An inducible DNA replication-cell division coupling mechanism inEscherichia coli. Nature (London) 1981; 30: 797–799.
Kuczuck MH, Benson PM, Heath H, Hayes AW. Evaluation of the mutagenic potential of mycotoxins usingSalmonella typhimurium andSaccharomyces cerevisiae. Mutation Res 1978; 53: 11–20.
Levin DE, Yamasaki E, Ames BN. A newSalmonella tester strain TA 97 for the detection of frameshift mutagens. A run of cytosines as a mutational hot-spot. Mutation Res 1982; 94: 315–330.
Little JW, Mount DW. The SOS regulatory system ofEscherichia coli. Cell 1982; 29: 11–22.
Mc Cann J, Choi E, Yamasaki E, Ames BN. Detection of carcinogens as mutagens in theSalmonella/microsomes test: Assay of 300 chemicals. Proc Natl Acad Sci USA 1975; 72: 5135–5139.
Marasas WFO, Van Rensburg SJ, Mirocha CJ. Incidence ofFusarium species and the mycotoxin deoxynivalenol and zearalenone, in corn produced in esophageal cancer areas in Transkei Agr Food Chem 1979; 27: 1108–1112.
Miller JH. Experiments in Molecular Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1972.
Moulé Y, Hermann M, Renault G. Negative resonse of PR toxin in theSalmonella typhimurium/microsome and sisterchromatid-exchange assay. Mutation Res 1982; 89: 203–207.
Polonelli L, Lauriola L, Morace G. Preliminary studies on the carcinogenic effect ofPenicillium roqueforti toxin (PR toxin) on rats. Mycopathologia 1982; 125–127.
Quillardet P, Huisman O, D'Ari R, Hofnung M. SOS chromotest, a direct assay of induction of an SOS function inEscherichia coli K-12 to measure genotoxicity. Proc Natl Acad Sci USA 1982; 79: 5971–5975.
Quillardet P, de Bellecombe C, Hofnung M. The SOS Chromotest, a colorimetric bacterial assay for genotoxins: validation study with 83 compounds. Mutation Res 1985; 147: 79–95.
Quillardet P, Hofnung M. The SOS Chromotest, a colorimetric bacterial assay for genotoxins: Procedures. Mutation Res 1985; 147: 65–78.
Radman M. SOS-repair hypothesis: Phenomenology of an inducible DNA repair which is accompagnied by mutagenesis. In: Hanawalt PC, Setlow RB, ed. Molecular mechanisms for repair of DNA. New York: Plenum Press, 1975; 355–367.
Rodricks JV, Hesseltine CW, Mehlan MA, Eds. Mycotoxins in human and animal health. Park Forest South: Pathotox 1977.
Schoental R. Health hazards of secondary metabolites ofFusarium. Microbiol Alim Nutr 1983; 1: 101–107.
Seino Y, Nagao M, Yahagi T, Hoshi A, Kawachi T, Sugimura T. Mutagenicity of several classes of antitumor agents toSalmonella typhimurium TA 98, TA 100 and TA 92. Cancer Res 1978; 38: 2148–2156.
Stark AA. Mutagenicity and carcinogenicity of mycotoxins: DNA binding as a possible mode of action. Annu Rev Microbiol 1980; 34: 235–263.
Ueno Y, Kubota K, Ito T, Nakamura Y. Mutagenicity of carcinogenic mycotoxins inSalmonella typhimurium. Cancer Res 1978; 38: 536–542.
Walker GC. Inducible DNA repair systems. Ann Rev Biochem 1985; 54: 425–457.
Wehner FC, Thiel PG, Van Rensburg SJ, Demasius IPC. Mutagenicity toSalmonella typhimurium of someAspergillus andPenicillium mycotoxins. Mutation Res 1978; 58: 193–203.
Witkin EM. The radiation sensitivity ofEscherichia coli B: a hypothesis relating filament formation and prophage induction. Proc Natl Acad Sci USA 1976; 57: 1275–1279.
Wong JJ, Hsieh DPH. Mutagenicity of aflatoxins related to their metabolism and carcinogenic potency. Proc Natl Acad Sci USA 1978; 73: 2241–2244.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Auffray, Y., Boutibonnes, P. Genotoxic activity of some mycotoxins using the SOS chromotest. Mycopathologia 100, 49–53 (1987). https://doi.org/10.1007/BF00769568
Issue Date:
DOI: https://doi.org/10.1007/BF00769568