Abstract
The healing of minimal skin lesions is usually obtained by epidermal migration and proliferation from peripheral wound margins. However, cutaneous grafts or reconstituted skin are necessary for severe injuries. Various models have recently been reproduced for this purpose. The aim of this work is to report the histopathologic evolution of burn lesions treated two years ago by autologous epidermis (Genzyme Tissue Repair, Boston, USA). Fifteen patients with severe burns (more than 80% of surface) have been treated. These observations have been based exclusively on biopsies of grafted wounds. Cultured epidermis is rapidly fully differentiated after grafting with temporary hyperplasia and normal strata. At 18 months, rete ridges formation is present only in young patients. Melanocytes and Langerhans' cells repopulated grafts rapidly. The use of cultured epidermis nowadays represents an important improvement in burn treatment.
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Abbreviations
- CEA:
-
cultured epidermal autologous sheets
- TBSA:
-
total burn surface area
References
Bell E, Ivarsson B, Merril C. Production of a tissue like structure by contraction of collagen lattices by human fibroblasts of different proliferative potentialin vitro. Proc Natl Acad Sci USA. 1979;76:1274–8.
Burke JF, Yannas IV, Quinby WC, Bondoc CC, Jung WK. Successful use of physiologically acceptable artificial skin in the treatment of extensive burn injury. Ann Surg. 1981;194:413–28.
Compton CC. Wound healing potential of cultured epithelium. Wounds. A compendium of clinical research and practice. 1993;5(2):97–110.
De Riols C, Charveron M, Eraki A, Bonafe JL, Neveux Y. Equivalent dermo-pasters on chronic ulceration. J Invest Dermatol. 1991;96:642.
Echinard C, Dantzer E, Damour O, Poinsignon F, Chabert B, Collombel C. Use of artificial dermis for skin loss repair. In: Tratamento dell ferrite. Monduzzi. 1987:85–7.
Green M, Kehinde O, Thomas J. Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. Proc Natl Acad Sci USA. 1979;76:5665–8.
Hansbrough JF, Boyce ST, Cooper ML et al. Burn wound closure with cultured autologous keratinocytes and fibroblasts attached to a collagen glycosaminoglycan substrate. J Am Med Assoc. 1989;262:2125–40.
Hull B, Finley R, Miller S. Coverage of full thickness burns with bilayered equivalents: a preliminary clinical trial. Surgery. 1990;107(5):496–502.
Kuroyanagi Y, Kenmochi M, Ishimara S et al. A cultured skin substitute composed of fibroblasts and keratinocytes with a collagen matrix: preliminary results of clinical trials. Ann Plast Surg. 1993;31(4):340–9.
Leigh IM, Purkis PE, Nausaria HA, Phillips JJ. Treatment of chronic venous ulcers with sheets of culturd allogenic keratinocytes. Br J Dermatol. 1987;117:591–7.
Madden MR, Finkelstein JL, Staiano-Coico L et al. Grafting of cultured allogenic epidermis on second and third degree burn wounds on 26 patients. J Trauma. 1986;26:955–62.
Neveux Y, Gervaise M, Guilbaud JL, Carsin H. Traitement des lésions de brûlures expérimentales par prothèse cutanée. Médecine et Armées. 1990;18(1):41–6.
Phillips TJ, Gilchrest BA. Cultured epidermal grafts in the treatment of leg ulcers. Adv Dermatol. 1990;5:33–50.
Rives JM. Cultured epithelial autografts. Clinical applications in extensive burns injuries. Communication at 9th Congress of the International Society for Burn Injuries (ISBI). 1994. Paris.
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Neveux, Y., Rives, J.M., Le Breton, C. et al. Clinical interest of cutaneous models reproducedin vitro for severe burn treatment: histopathological and ultrastructural study. Cell Biol Toxicol 11, 173–178 (1995). https://doi.org/10.1007/BF00756520
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DOI: https://doi.org/10.1007/BF00756520