Abstract
Intracellular pressure injection of cyclic AMP induces a slow voltage-dependent inward current in some neurons of Aplysia californica.The time course, voltage dependence, and ionic sensitivities of this response are nearly identical to those of the voltage-dependent calcium current induced by serotonin in the same preparation. The response to cyclic AMP is unaffected by changes in the extracellular concentration of chloride or potassium. The current is slowly but minimally reduced by a sodium-free solution. The calcium channel blocker, cadmium, blocks the current elicited by injection of cyclic AMP. The data presented here suggest that cyclic AMP can induce a voltage-dependent calcium current.
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References
Ascher, P., Kunze, D., and Neild, T. O. (1976). Chloride distribution inAplysia neurons.J. Physiol. (Lond) 256441–464.
Baker, P. F. (1976). Regulation of intracellular Ca and Mg in squid axons.Fed. Proc. 352589–2595.
Berridge, M. J. (1975). The interaction of cyclic nucleotides and calcium in the control of cellular activity.Adv. Cyclic Nucleotide Res. 61–98.
Berridge, M. J. (1979). Modulation of nervous activity by cyclic nucleotides and calcium. In F. O. Schmitt and F. G. Worden (eds.),The Neurosciences Fourth Study Program, MIT Press, Cambridge, Mass., pp. 873–889.
Blaustein, M. P. (1976). The ins and outs of calcium transport in squid axons: internal and external ion activation of calcium efflux.Fed. Proc. 352574–2578.
Bloom, F. E. (1979). Cyclic nucleotides in central synaptic function.Fed. Proc. 382203–2207.
Carafoli, E., and Crompton, M. (1978). The regulation of intracellular calcium.Current Topics in Membranes and Transport 10151–216.
Cedar, H., and Schwartz, J. H. (1972). Cyclic adenosine monophosphate in the nervous system ofAplysia californica. II. Effect of serotonin and dopamine.J. Gen. Physiol. 60570–587.
Cheung, W. Y. (1980). Calmodulin plays a pivotal role in cellular regulation.Science 20719–27.
DiPolo, R. (1978). Ca pump driven by ATP in squid axons.Nature 274390–392.
Eckert, R., and Lux, H. D. (1976). A voltage-sensitive persistent calcium conductance in neuronal somata ofHelix.J. Physiol. (Lond.) 254129–151.
Erulkar, S. D., and Fine, A. (1979). Calcium in the nervous system.Rev. Neurosci. 4179–232.
Frazier, W. T., Kandel, E. R., Kupfermann, I., Waziri, R., and Coggeshall, R. E. (1967). Morphological and functional properties of identified neurons in the abdominal ganglion ofAplysia californica.J. Neurophysiol. 301288–1351.
Gerschenfeld, H. M., and Paupardin-Tritsch, D. (1974). Ionic mechanisms and receptor properties underlying the responses of molluscan neurones ot 5-hydroxytryptamine.J. Physiol. (Lond.) 243427–456.
Greengard, P. (1978).Cyclic Nucleotides, Phosphorylated Proteins and Neuronal Function, Raven Press, New York.
Hagiwara, S. (1973). Ca spike.Adv. Biophys. 471–102.
Johnston, D. (1976). Voltage clamp reveals basis for calcium regulation of bursting pacemaker potential inAplysia neurons.Brain Res. 107418–423.
Kandel, E. R., Brunelli, M., Byrne, J., and Castellucci, V. (1976). A common presynaptic locus for the synaptic changes underlying short-term habituation and sensitization of the gill withdrawal reflex inAplysia.Cold Spring Harbor Symp. Quant. Biol. 40465–482.
Katz, B., and Miledi, R. (1967) The timing of calcium action during neuromuscular transmission.J. Physiol. (Lond.) 189535–544.
Kebabian, J. W. (1977). Biochemical regulation and physiological significance of cyclic nucleotides in the nervous systems.Adv. Cyclic Nucleotide Res. 8421–508.
Kehoe, J. (1972). Ionic mechanisms of a two-component cholinergic inhibition inAplysia neurones.J. Physiol. (Lond.) 25585–114.
Klein, M., and Kandel, E. R. (1978). Presynaptic modulation of voltage-dependent Ca++ current: mechanism for behavioral sensitization inAplysia californica. Proc. Natl. Acad. Sci. USA753512–3516.
Kostyuk, P. G., and Krishtal, O. A. (1977a). Separation of sodium and calcium currents in the somatic membrane of mollusc neurones.J. Physiol. (Lond.) 279545–568.
Kostyuk, P. G., and Krishtal, O. A. (1977b). Effects of calcium and calcium-chelating agents on the inward and outward current in the membrane of mollusc neurones.J. Physiol. (Lond.) 279569–580.
McCaman, R. E., McKenna, D. G., and Ono, J. K. (1977). A pressure system for intracellular and extracellular ejections of picoliter volumes.Brain Res. 136141–147.
Meech, R. W. (1972). Intracellular calcium injection causes increased potassium conductance inAplysia nerve cells.Comp. Biochem. Physiol. 42A493–499.
Meech, R. W., and Standen, N. B. (1975). Potassium activation inHelix aspersa neurones under voltage clamp: A component mediated by calcium influx.J. Physiol. (Lond.) 249211–239.
Miyamoto, M. D., and Breckenridge, B. McL. (1974). A cyclic adenosine monophosphate link in the catecholamine enhancement of transmitter release at the neuromuscular junction.J. Gen. Physiol. 63609–624.
Nathanson, J. A. (1977). Cyclic nucleotides and nervous system function.Physiol. Rev. 57157–256.
Pellmar, T. A transmitter-induced calcium current.Fed. Proc. (in press).
Pellmar, T. C., and Carpenter, D. O. (1979). Voltage-dependent calcium current induced by serotonin.Nature 277483–484.
Pellmar, T. C., and Carpenter, D. O. (1980). Serotonin induces a voltage-sensitive calcium current in neurons ofAplysia californica.J. Neurophysiol. 44423–439.
Pellmar, T. C., and Wilson, W. A. (1977). Unconventional serotonergic excitation inAplysia.Nature 26976–78.
Rasmussen, H., and Goodman, D. B. P. (1977). Relationship between calcium and cyclic nucleotides in cell activation.Physiol. Rev. 57421–509.
Shimahara, T., and Tauc, L. (1977). Cyclic AMP induced by serotonin modulates the activity of an identified synapse inAplysia by facilitating the active permeability to calcium.Brain Res. 127168–172.
Standaert, F. G., and Dretchen, K. L. (1979). Cyclic nucleotides and neuromuscular transmission.Fed. Proc. 382183–2192.
Takahashi, K., and Yoshii, M. (1978). Effects of internal free calcium upon the sodium and calcium channels in the tunicate egg analysed by the internal perfusion technique.J. Physiol. (Lond.) 279519–549.
Thompson, S. H. (1977). Three pharmacologically distinct potassium channels in molluscan neurones.J. Physiol. (Lond.) 265465–488.
Treistman, S. N., and Levitan, I. B. (1976). Alteration of electrical activity in molluscan neurones by cyclic nucleotides and peptide factors.Nature 26162–64.
Wilson, W. A., and Goldner, M. M. (1974). Voltage clamping with a single microelectrode.J. Neurobiol. 6411–422.
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Pellmar, T.C. Ionic mechanism of a voltage-dependent current elicited by cyclic AMP. Cell Mol Neurobiol 1, 87–97 (1981). https://doi.org/10.1007/BF00736041
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DOI: https://doi.org/10.1007/BF00736041