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Generation of Stable Catharanthus roseus Hairy Root Lines with Agrobacterium rhizogenes

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Plant Secondary Metabolism Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2469))

Abstract

Agrobacterium rhizogenes is the bacterial agent that causes hairy root disease in dicots and is purposefully engineered for the development of transgenic hairy root cultures. Due to their genetic and metabolic stability, hairy root cultures offer advantages as a tissue culture system for investigating the function of transgenes and as a production platform for specialized metabolites or proteins. The process for generating hairy root cultures involves first infecting the explant with A. rhizogenes, excising and eliminating A. rhizogenes from the emerging hairy roots, selecting for transgenic hairy roots on plates containing the selective agent, confirming genomic integration of transgenes by PCR, and finally adapting the hairy roots in liquid media. Here we provide a detailed protocol for developing and maintaining transgenic hairy root cultures of our medicinal plant of interest, Catharanthus roseus.

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Author notes

  1. Krystyna K. F. Traverse and Samuel Mortensen contributed equally with all other contributors.

Authors and Affiliations

  1. Department of Chemical Engineering, Northeastern University, Boston, MA, USA

    Krystyna K. F. Traverse, Noreen F. Rizvi & Carolyn W. T. Lee-Parsons

  2. Department of Biology, Northeastern University, Boston, MA, USA

    Samuel Mortensen & Hope Danison

  3. Department of Bioengineering, Northeastern University, Boston, MA, USA

    Juliet G. Trautman

  4. Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA

    Carolyn W. T. Lee-Parsons

Authors
  1. Krystyna K. F. Traverse
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  2. Samuel Mortensen
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  3. Juliet G. Trautman
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  4. Hope Danison
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  5. Noreen F. Rizvi
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  6. Carolyn W. T. Lee-Parsons
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Corresponding author

Correspondence to Carolyn W. T. Lee-Parsons .

Editor information

Editors and Affiliations

  1. Center for Biotechnology and Department of Botany, Plant Physiology Laboratory, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

    Arthur Germano Fett-Neto

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Traverse, K.K.F., Mortensen, S., Trautman, J.G., Danison, H., Rizvi, N.F., Lee-Parsons, C.W.T. (2022). Generation of Stable Catharanthus roseus Hairy Root Lines with Agrobacterium rhizogenes . In: Fett-Neto, A.G. (eds) Plant Secondary Metabolism Engineering. Methods in Molecular Biology, vol 2469. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2185-1_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2185-1_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2184-4

  • Online ISBN: 978-1-0716-2185-1

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