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Species-specific expression of sialosyl-Lex on polymorphonuclear leukocytes (PMN), in relation to selectin-dependent PMN responses

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Abstract

Sialosyl-Lex (SLex) and its positional isomer sialosyl-Lea are the epitopes recognized by the lectin domain of E- and P-selectins. Expression of SLex in polymorphonuclear leukocytes (PMN) plays an important role in recruitment of these cells at sites of inflammation through activation of selectins. We studied expression of SLex in PMN of seven mammalian species in comparison with that in humans. Only PMN of humans (no other species) expressed SLex or other lacto-series epitopes such as Lex or Ley. The observed absence of these epitopes in rat PMN seems inconsistent with recent reports that the lung inflammation process in a rat model is inhibited by perfusion of SLex oligosaccharide (Mulligan MS,et al. (1993a)Nature 364:149; (1993b)J Exp Med 178:623). Rat selectins may be able to recognize SLex, even though this epitope is absent in rat PMN.

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Abbreviations

FITC:

fluorescein isothiocyanate

mAb:

monoclonal antibody

PMN:

polymorphonuclear leukocytes

SLea :

sialosyl-Lea antigen

SLex :

sialosyl-Lex antigen

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Authors and Affiliations

  1. The Biomembrane Institute, 201 Elliott Avenue West, 98119, Seattle, WA, USA

    Kazunori Ito, Kazuko Handa & Sen-Itiroh Hakomori

  2. Departments of Pathobiology and Microbiology, University of Washington, 98195, Seattle, WA, USA

    Kazunori Ito, Kazuko Handa & Sen-Itiroh Hakomori

Authors
  1. Kazunori Ito
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  2. Kazuko Handa
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  3. Sen-Itiroh Hakomori
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Ito, K., Handa, K. & Hakomori, SI. Species-specific expression of sialosyl-Lex on polymorphonuclear leukocytes (PMN), in relation to selectin-dependent PMN responses. Glycoconjugate J 11, 232–237 (1994). https://doi.org/10.1007/BF00731223

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  • DOI: https://doi.org/10.1007/BF00731223

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