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Bi-antennary oligo-(N-acetyllactosamino)glycans of I-type are galactosylated preferentially at the GlcNAcβ1-6Gal linked arms by α1,3-galactosyltransferase of bovine thymus

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Abstract

α1,3-Galactosylation of radiolabelled bi-antennary acceptors Galβ1-4GlcNAcβ1-3(Galβ1-4GlcNAcβ1-6)Gal-R (R=1-OH, β1-4GlcNAc or β1-4Glc) with bovine thymus α1,3-galactosyltransferase was studied. At all stages of the reactions the three acceptors reacted faster at the 1 → 6 linked arm than at the 1 → 3 linked branch. Hence, in addition to the doubly α1,3-galactosylated products, practically pure Galβ1-4GlcNAcβ1-3(Galα1-3Galβ1-4GlcNAcβ1-6)Gal-R could be obtained from the three acceptors in reactions that had proceeded to near completion. The isomeric mono-α1,3-galactosylated products were identified by using exoglycosidases to remove the branches unprotected by α1,3-galactoses and by subsequently identifying the resulting linear glycans chromatographically.

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Abbreviations

Gal:

d-galactose

GlcNAc:

N-acetyl-d-glucosamine

Lac:

lactose

LacNAc:

Galβ1-4GlcNAc

MH:

maltoheptaose

MP:

maltopentaose

MT:

maltotriose

MTet:

maltotetraose

WGA:

wheat germ agglutinin

3′:

position 3 of the galactose unit of LacNAc or Lac

6′:

position 6 of the galactose unit of LacNAc or Lac

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Authors and Affiliations

  1. Institute of Biotechnology and Department of Biochemistry, University of Helsinki, Valimotie 7, 00380, Helsinki, Finland

    Antti Seppo, Leena Penttilä, Anne Leppänen, Hannu Maaheimo, Ritva Niemelä, Jari Helin & Ossi Renkonen

  2. Laboratoire de Chimie Biologique and U.M.R. No. 111 du C.N.R.S., Université des Sciences et Technologies de Lille, 59655, Villeneuve d'Ascq Cedex, France

    Jean-Michel Wieruszeski

Authors
  1. Antti Seppo
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  2. Leena Penttilä
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  3. Anne Leppänen
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  4. Hannu Maaheimo
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  5. Ritva Niemelä
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  6. Jari Helin
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  7. Jean-Michel Wieruszeski
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  8. Ossi Renkonen
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Seppo, A., Penttilä, L., Leppänen, A. et al. Bi-antennary oligo-(N-acetyllactosamino)glycans of I-type are galactosylated preferentially at the GlcNAcβ1-6Gal linked arms by α1,3-galactosyltransferase of bovine thymus. Glycoconjugate J 11, 217–225 (1994). https://doi.org/10.1007/BF00731221

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  • DOI: https://doi.org/10.1007/BF00731221

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