Abstract
Leaf explants ofKalanchoe laciniata were cocultivated for different days (2, 4, 6 and 8 days) with disarmedAgrobacterium tumefaciens strains A208SE, GV3111SE and EHA101 carring a binary vector pROA93. The vector contains a cauliflower mosaic virus 35S promotor which drives the coding sequence of neomycin phosphotransferase II (NPT-II) in one direction and β-glucuronidase (GUS) in the opposite direction. Prolonged cocultivation (6 days) resulted in a marked increase of GUS gene transient expression, in terms of, the number of explants with transformed cells (up to 100%) and the percent area of transformed tissue (∼ 50%). Explants cocultivated for 6–7 days showed a dramatic increase in the frequency of stable transformation and 75–80% of the inoculated explants produced transgenic plants. Cocultivation with the nopaline strain A208SE for 7 days gave as high as 10 transgenic plants per explant.
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Abbreviations
- GUS:
-
β-glucuronidase
- NPT:
-
neomycin phosphotransferase
- MS:
-
Murashige and Skoog (1962)
- BA:
-
6-benzylaminopurine
- IAA:
-
indol-3-acetic acid
- KT:
-
kinetin
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Communicated by R. N. Beachy
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Jia, SR., Yang, MZ., Ott, R. et al. High frequency transformation ofKalanchoe laciniata . Plant Cell Reports 8, 336–340 (1989). https://doi.org/10.1007/BF00716668
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DOI: https://doi.org/10.1007/BF00716668