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Cyclic nucleotide metabolism in the sympathetic ganglion

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Summary

  1. 1.

    The synaptic processes controlling cyclic nucleotide metabolism in the rat superior cervical ganglion were examined through electrical stimulation of preganglionic and postganglionic nerves.

  2. 2.

    Both cyclic AMP and cyclic GMP were measured in each ganglion. Cyclic AMP was increased twofold by preganglionic but not postganglionic stimulation for 30 sec.

  3. 3.

    Carbachol also increased cyclic AMP twofold. These elevations were calcium dependent and atropine sensitive, consistent with a reliance on muscarinic transmission. However, atropine did not inhibit the elevation of cyclic AMP following preganglionic stimulation for 60 sec.

  4. 4.

    A much larger increase in cyclic AMP could be induced by stimulation ofβ-adrenergic receptors, but this mechanism did not appear to be invoked by nervous activity.

  5. 5.

    Cyclic GMP levels were increased sixfold by preganglionic stimulation, twofold by postganglionic stimulation, and twofold by carbachol. All of these effects on cyclic GMP levels were calcium sensitive.

  6. 6.

    Atropine didnot inhibit the effect of 30-sec preganglionic stimulation on cyclic GMP. However, atropine blocked the cyclic GMP increases induced by carbachol or by postganglionic stimulation and partially inhibited the increase following 60-sec preganglionic stimulation.

    Thus, synaptic activity alters the levels of both cyclic AMP and cyclic GMP in the rat ganglion, but more than one process is involved and these processes appear to be considerably different.

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Authors and Affiliations

  1. Division of Neurosciences, City of Hope Research Institute, 91010, Duarte, California, USA

    Clark A. Briggs, Gary J. Whiting & Donald A. McAfee

  2. Department of Anatomy and Neurobiology, University of Vermont School of Medicine, 05405, Burlington, Vermont, USA

    Marjorie A. Ariano

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  1. Clark A. Briggs
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  2. Gary J. Whiting
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  3. Marjorie A. Ariano
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  4. Donald A. McAfee
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Briggs, C.A., Whiting, G.J., Ariano, M.A. et al. Cyclic nucleotide metabolism in the sympathetic ganglion. Cell Mol Neurobiol 2, 129–141 (1982). https://doi.org/10.1007/BF00711078

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  • DOI: https://doi.org/10.1007/BF00711078

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