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An ultrastructural comparison of the cell envelopes of selected strains of Nocardia asteroides and Nocardia brasiliensis

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Abstract

Growth curves were determined for three strains each ofNocardia asteroides andNocardia brasiliensis. Two strains ofN. brasiliensis and one strain ofN. asteroides had longer lag periods of growth than the remaining three strains. All strains had generation times of approximately 5.5 hours.

The ultrastructure of the cell envelope of eachNocardia strain in early stationary phase growth was also examined. All the strains had typical trilaminar cell walls and cell membranes. The thickness of the cell wall layers, especially the inner peptidoglycan layer, varied from strain to strain. The inner layer of two strains ofN. brasiliensis and one strain ofN. asteroides was 12 nm or more in thickness, while that of the remaining three strains was 7 nm thick. These observed differences in growth patterns and/or thickness of the cell wall layers could be correlated to the varying degress of virulence as well as the divergent pathologies exhibited by these organisms.

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Author notes

  1. Geoffrey A. Land

    Present address: Clinical Microbiology Lab, Univ. of Cincinnati, Med. Ctr., 122 Mont Reid Pauillion, 45267, Cincinnati, Ohio, USA

Authors and Affiliations

  1. Wadley Institutes of Molecular Medicine, 9000 Harry Hines, 75235, Dallas, Texas, USA

    Teresa A. Beadles,  Geoffrey A. Land & David J. Knezek

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  1. Teresa A. Beadles
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  2. Geoffrey A. Land
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  3. David J. Knezek
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Beadles, T.A., Land, G.A. & Knezek, D.J. An ultrastructural comparison of the cell envelopes of selected strains of Nocardia asteroides and Nocardia brasiliensis. Mycopathologia 70, 25–32 (1980). https://doi.org/10.1007/BF00704318

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