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Characterization of an established human malignant glioma cell line: LN-18

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Summary

A human malignant glioma cell line, LN-18, has been established in monolayer culture and subcultured for more than 115 passages. LN-18 cells grow in vitro as bipolar or stellate cells with pleomorphic nuclei, have a doubling time of about 72 h and a plating efficiency of 3%. The glial nature of these cells has been assessed by ultrastructural examination. The synthesis of glial fibrillary acidic and S-100 proteins could not be demonstrated, although the initial biopsy tissue and the early cultures were positive for the former. The presence of Ia-like antigens on the surface of these cells was demonstrated using allo and xeno antisera. LN-18 cells were also shown to synthesize large quantities of fibronectin. The injection of LN-18 cells into nude mice induced the formation of solid tumor masses that could be retransplanted every 3 weeks and showed a morphology comparable to that of the initial biopsy. Karyotype analysis revealed the presence of three marker chromosomes, constantly present before and after hetero-transplantation.

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Abbreviations

GFA:

glial acidic fibrillary protein

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Authors and Affiliations

  1. Neurosurgical Service, CHUV, CH-1011, Lausanne, Switzerland

    A. C. Diserens, N. de Tribolet, A. Martin-Achard & J. F. Schnegg

  2. Department of Medical Genetics, CHUV, CH-1011, Lausanne, Switzerland

    A. C. Gaide

  3. Unit of Human Cancer Immunology, Lausanne Branch, Ludwig Institute for Cancer Research, Epalinges, Switzerland

    S. Carrel

Authors
  1. A. C. Diserens
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  2. N. de Tribolet
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  3. A. Martin-Achard
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  4. A. C. Gaide
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  5. J. F. Schnegg
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  6. S. Carrel
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Additional information

This work was supported by grants from the Swiss League Against Cancer and the Swiss National Foundation for Scientific Research CNP: 2′3′-cyclic nucleotide 3′-phosphodiesterase

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Diserens, A.C., de Tribolet, N., Martin-Achard, A. et al. Characterization of an established human malignant glioma cell line: LN-18. Acta Neuropathol 53, 21–28 (1981). https://doi.org/10.1007/BF00697180

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  • DOI: https://doi.org/10.1007/BF00697180

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