Summary
The responses of testes, body weight, and pelage colour to short and long photoperiods in winter were determined in adult male hamsters with and without the implantation of melatonin. Animals in winter condition, with involuted testes and winter pelage, were kept at 20 °C under conditions of either long (16 h per day) or short (8 h per day) photoperiods beginning on 2 January. In each condition one group was implanted three times at weekly intervals with melatonin in beeswax, a control group was implanted with beeswax only, and another control group was left untreated. A further control group remained in natural day light. After 37 days testes of the control groups in long photoperiods had reached summer condition, while the group treated with melatonin was delayed in testicular development, and closely resembled both the three shortday groups and the group kept in natural daylight (Fig. 2–4). In short photoperiods there was no difference between the group treated with melatonin and the two control groups. All groups showed some testis development as compared to animals killed at the beginning of the experiment. Hamsters kept under natural daylight showed a marked annual cycle of body weight which closely paralleled gonadal activity (Fig. 5). In the experimental groups there was a corresponding increase in body weight paralleling testicular development (Fig. 6). The two control groups in long photoperiods had a significantly higher increase in body weight than all other groups, while there were no significant differences between the groups treated with melatonin, the two short-day groups and the group under natural daylight. Testis size at the end of the experiment was highly correlated with increase in body weight (Fig. 7, and Table 1).
Molt into summer pelage had started in all groups at the end of the experiment. Colour change was most advanced in the two control groups under long photoperiods, while the long-day group treated with melatonin resembled the short-day groups (Figs. 9 and 10).
It is concluded that the change in physiological state from winter to summer is based on an endogenous mechanism, which is accelerated by long photoperiods, and that melatonin inhibits or greatly diminishes this acceleration while it does not inhibit spontaneous development towards summer condition.
Similar content being viewed by others
References
Aschoff, J.: Jahresperiodik der Fortpflanzung beim Warmblüter. Studium gen.8, 742–776 (1955)
Benson, B., Matthews, M. J., Rodin, A. E.: A melatonin-free extract of bovine pineal with antigonadotropic activity. Life Sci.10, 607–612 (1971)
Eichler, V. B., Moore, R. Y.: Pineal hydroxyindole-O-methyltransferase and gonadal responses to blinding or continuous darkness blocked by pineal denervation in the male hamster. Neuroendocrinology8, 81–85 (1971)
Elliot, J. A., Stetson, M. H., Menaker, M.: Regulation of testis function in golden hamsters: a circadian clock measures photoperiodic time. Science178, 771–773 (1972)
Ellis, L. C.: The direct action of melatonin and serotonin on testicular androgen production in vitro. J. Reprod. Fertil.18, 159 (1969)
Evans, D. M.: Seasonal variations in the body composition and nutrition of the voleMicrotus agrestis. J. Anim. Ecol.42, 1–18 (1973)
Farner, D. S.: Comparative physiology: photoperiodicity. Ann. Rev. Physiol.23, 71–96 (1961)
Figala, J., Hoffmann, K., Goldau, G.: Zur Jahresperiodik beim Dsungarischen ZwerghamsterPhodopus sungorus Pallas. Oecologia (Berl.)12, 89–118 (1973)
Gaston, S., Menaker, M.: Photoperiodic control of hamster testis. Science158, 925–928 (1967)
Gwinner, E.: A comparative study of circannual rhythms in warblers. In: Biochronometry (M. Menaker, ed.), p. 405–427. Washington, D. C.: Nat. Acad. Sci. 1971
Herbert, J.: The role of the pineal gland in the control by light of the reproductive cycle of the ferret. In: The pineal gland (G. E. W. Wolstenholme, J. Knight, eds.), p. 303–327. Edinburgh: Churchill Livingstone 1971
Herbert, J.: Initial observations on pinealectomized ferrets kept for long periods in either daylight or artificial illumination. J. Endocr.55, 591–597 (1972)
Huffman, R. J., Hester, R. J., Towns, C.: Effect of light and temperature on the endocrine system of the golden hamster (Hesocricetus auratus Waterhouse). Comp. Biochem. Physiol.15, 525–533 (1965)
Kappers, J. A.: The mammalian pineal organ. J. Neuro-Viscer. Relat., Suppl. IX, 140–184 (1969)
Kenagy, G. J.: Daily and seasonal patterns of activity and energetics in a heteromyid rodent community. Ecology (in press, 1973)
Khateeb, A. al, Johnson, E.: Seasonal changes of pelage in the vole (Microtus agrestis). I. Correlation with changes in the endocrine glands. Gen. comp. Endocr.16, 217–228 (1971 a).
Khateeb, A. al, Johnson, E.: Seasonal changes of pelage in the vole (Microtus agrestis). II. The effect of daylength. Gen. comp. Endocr.16, 229–235 (1971b)
Kinson, G. A., Liu, Ch. -Ch.: Testicular responses to melatonin and serotonin implanted peripherally in immature rats. Life Sci.12, 173–184 (1973)
Pengelley, E. T.: The relation of external conditions to the onset and termination of hibernation and estivation. In: Mammalian hibernation III (K. C. Fisher, A. R. Dawe, C. P. Lyman, E. Schönbaum, F. E. South, eds.), p. 1–29. Edinburgh-London: Oliver & Boyd 1967
Quay, W. B.: The role of the pineal gland in environmental adaptation. In: Physiology and pathology of adaptation mechanisms (E. Bajusz, ed.), p. 508–550. Oxford-New York: Pergamon Press 1969
Redding, T. W., Nair, R. M. G., Kastin, A. J.: Physiological studies with14C-melanocyte stimulating hormone-inhibiting factor (MIF). Fed. Proc.31, 811 (1972)
Redding, T. W., Schally, A. V.: The distribution, half-life, and excretion of tritiated hormone (LH-RH) in rats. Life Sci.12, 23–32 (1973)
Reiter, R. J.: Pineal-gonadal relationships in male rodents. In: Progress in endocrinology (C. Gual, ed.), p. 631–636. Amsterdam: Excerpta Medica 1969
Reiter, R. J.: The role of the pineal in reproduction. In: Reproductive biology (H. Balin, S. Glasser, eds), p. 71–114. Amsterdam: Excerpta Medica 1972
Reiter, R. J.: Evidence for pineal-induced seasonal changes in reproductive physiology of male hamsters kept under natural environmental conditions. Fed. Proc.32, 251 (1973)
Reiter, R. J., Fraschini, F.: Endocrine aspects of the mammalian pineal gland: a review. Neuroendocrinology5, 219–255 (1969)
Reiter, R. J., Sorrentino, S.: Reproductive effects of the mammalian pineal. Amer. Zoologist.10, 247–258 (1970)
Rust, C. C.: Hormonal control of pelage cycles in the short tailed weasel (Mustela erminea bangsi). Gen. comp. Endocr.5, 222–231 (1965)
Rust, C. C., Meyer, R. K.: Effect of pituitary autografts on hair color in the short-tailed weasel. Gen. comp. Endocr.11, 548–551 (1968)
Rust, C. C., Meyer, R. K.: Hair color, molt, and testis size in male, short-tailed weasels treated with melatonin. Science165, 921–922 (1969)
Wurtman, R. J.: Effects of light and visual stimuli on endocrine function. In: Neuroendocrinology, vol. 2 (L. Martini, W. F. Ganong, eds.), p. 20–59. New York-London: Academic Press 1967
Wurtman, R. J., Axelrod, J., Kelly, D. E.: The pineal. New York-London: Academic Press 1968
Author information
Authors and Affiliations
Additional information
I am most grateful to Frau Dipl.-Biol. M. Güttinger for the histological work, and to Prof. K.-H. Frömming, Pharmazeutisches Institut, Freie Universität Berlin, for the determination of melatonin content in the implants as well as for technical advice. Mr. H. Biebach, Mr. and Mrs. Goldau, Dr. E. Gwinner, and Dr. and Mrs. I. F. Spellerberg assisted in colour scoring; Miss M. Wendon, and Drs. J. Aschoff, E. Gwinner, G. Heldmaier, G. J. Kenagy, and H. Underwood critically read the manuscript and made many helpful suggestions. Thanks are also due to Dr. W. B. Quay, Dept. of Biology, Berkeley, and Dr. G. Heldmaier, for much valuable information and many helpful suggestions on pineal physiology and on technical points.
Rights and permissions
About this article
Cite this article
Hoffmann, K. The influence of photoperiod and melatonin on testis size, body weight, and pelage colour in the Djungarian hamster (Phodopus sungorus). J. Comp. Physiol. 85, 267–282 (1973). https://doi.org/10.1007/BF00694233
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00694233