Summary
Characteristics of autofluorescence emission spectra are reported from neuromelanin autofluorescence, after paraffin wax-embedded sections of human substantia nigra were bleached by exposure to 15% hydrogen peroxide for 24h. The characteristics of a pigment's emission spectrum probably reflect its composition, and the results are compared with autofluorescence emission spectra from examples of bleached “lipofuscin” and bleached “ceroid”. (In the present study “bleached” implies that the tissue was exposed to hydrogen peroxide, whether or not a change in pigment colour was observed.)
The spectral characteristics of bleached neuromelanin autofluorescence were distinguished from those of bleached lipofuscin but not from examples of bleached ceroid; thus, despite similarities between neuromelanin and lipofuscin, neuromelanin resembles some examples of ceroid but not lipofuscin in respect of the properties investigated. The process of neuromelanin formation may be related to pathogenic mechanisms in the ceroidoses.
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References
Ambani LM, Jhung JW, Edelstein SM, Van Woert MH (1977) Quantification of melanin in hepatic and cardiac lipofuscin. Experientia 33:296–298
Barden H (1969) The histochemical relationship of neuromelanin and lipofuscin. J Neuropathol Exp Neurol 29:419–441
Barden H (1979) Acid fast staining of oxidized neuromelanin and lipofuscin in the human brain. J Neuropathol Exp Neurol 38:453–461
Barden H (1980) Interference filter microfluorometry of neuromelanin and lipofuscin in human brain. J Neuropathol Exp Neurol 39:598–605
Dowson JH (1982a) Neuronal lipofuscin accumulation in ageing and Alzheimer dementia: A pathogenic mechanism? Br J Psychiatry 140:142–148
Dowson JH (1982b) The evaluation of autofluorescence emission spectra derived from neuronal lipopigment. J Microsc 128:261–270
Dowson JH (1983) Autofluorescence emission spectra of neuronal lipopigment in a case of adult-onset ceroidosis (Kufs' disease). Acta Neuropathol (Berl) 59:241–245
Dowson JH, Armstrong D, Koppang N, Lake BD, Jolly RD (1982) Autofluorescence emission spectra of neuronal lipopigment in animal and human ceroidoses (ceroid-lipofuscinoses). Acta Neuropathol (Berl) 58:125–156
Dowson JH, Harris SJ (1981) Quantitative studies of the autofluorescence derived from neuronal lipofuscin. J Microsc 123:249–258
Feeney L (1978) Lipofuscin and melanin of human retinal pigment epithelium. Invest Ophthalmol Vis Sci 17:583–599
Mann DMA, Yates PO (1974) Lipoprotein pigments — their relationship to ageing in the human nervous system. Brain 97: 489–498
Mann DMA, Yates PO, Barton CM (1977) Neuromelanin and RNA in cells of substantia nigra. J Neuropathol Exp Neurol 36:379–383
Roy S, Wolman L (1969) Ultrastructural observations in parkinsonism. J Pathol 99:39–44
Seitelberger F (1971) Pigmentary disorders In: Minckler J (ed) Pathology of the nervous system. McGraw-Hill, New York, pp 1324–1338
Siegel S (1956) In: Nonparametric statistics. McGraw-Hill, New York San Francisco London, pp 68–127
Zeman W (1976) The neuronal ceroid-lipofuscinoses. In: Zimmerman HH (ed) Progress in neuropathology, vol 111. Grune and Stratton, New York San Francisco London, pp 1–44
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Dowson, J.H. A comparison of autofluorescence emission spectra of bleached neuromelanin in non-diseased substantia nigra with spectra of other intraneuronal pigments in non-diseased and diseased tissue. Acta Neuropathol 61, 196–200 (1983). https://doi.org/10.1007/BF00691985
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DOI: https://doi.org/10.1007/BF00691985