Summary
Flow cytometric techniques were used to compare the DNA content, size and viability of meningioma cells obtained directly from surgical specimens with the same cells after a period of culture. Cells isolated from the original meningiomas and cells in primary culture were similar with regard to size and DNA content, regardless of the histologic subclassification of tumor. The cell populations were essentially diploid with a small proportion of tetraploid cells. Viable cells were smaller and more uniform in size than the nonviable cells. An increase in the number of cells having an elevated DNA content was seen with cultures repeatedly transferred. The latter results suggest that any transfer of information from long-term cultured meningioma cells to the in vivo situation must be done with caution.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adams, L. R.: Staining for the cytograph and cytofluorograf. J. Histochem. Cytochem.25, 965–975 (1977)
Bellemann, R., Gebhard, R., Mecke, D.: An improved method for the isolation of hepatocytes from liver slices. Selective removal of trypan blue-dyeable cells. Anal. Biochem.81, 408–415 (1977)
Costero, I., Pomerat, C. M., Barroso-Moguel, R., et al.: Tumors of the human nervous system in tissue culture. II. An analysis of fibroblastic activity in meningiomas. J. Natl. Cancer Inst.15, 1341–1365 (1955a)
Costero, I., Pomerat, C. M., Jackson, J. J., et al.: Tumors of the human nervous system in tissue culture. I. The cultivation and cytology of meningioma cells. J. Natl. Cancer Inst.15, 1319–1338 (1955b)
Crissman, H. A., Steinkamp, J. A.: Rapid, simultaneous measurement of DNA, protein and cell volume in single cells from large mammalian cell populations. J. Cell. Biol.59, 766–771 (1973)
Frederiksen, P., Reske-Nielsen, E., Bichel, P.: Flow cytometry in tumours of the brain. Acta Neuropathol. (Berl.)41, 179–183 (1978)
Greenberg, S. B., Grove, G. L., Crisfalo, V. J.: Cell size in aging monolayer cultures. In Vitro13, 297–300 (1977)
Horan, P. K., Wheeles, LL., Jr.: Quantitative single cell analysis and sorting. Science198, 149–157 (1977)
Hoshino, T., Nomura, K., Gray, J. H., Wilson, C. B.: Flow microfluorometric studies of brain and brain tumors. J. Neuropathol. Exp. Neurol.35, 363 (1976) (abst.)
Hoshino, T., Nomura, K., Wilson, C. B.: The distribution of nuclear DNA from human brain tumor cells. J. Neuropathol. Exp. Neurol.36, 606 (1977) (abst.)
Koss, L. G., Wolley, R. G., Schreiber, K., et al.: Flow-microfluorometric analysis of nuclei isolated from various normal and malignant human epithelial tissues. A preliminary report. J. Histochem. Cytochem.25, 565–572 (1977)
Krishan, A.: Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. J. Cell Biol.66, 188–193 (1975)
Melamed, M. R. Kamentsky, L. A.: Automated cytology. Int. Rev. Exp. Pathol.14, 205–295 (1975)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kajikawa, H., Kawamoto, K., Herz, F. et al. Flow-through cytometry of meningiomas and cultured meningioma cells. Acta Neuropathol 44, 183–187 (1978). https://doi.org/10.1007/BF00691065
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00691065